Reagent and laboratory contamination can critically impact sequence-based microbiome analyses

BMC Biology

Volumn 12, Issue 1, 2014, Pages

  Salter, Susannah J ^a   Cox, Michael J ^b   Turek, Elena M ^b   Calus, Szymon T ^c   Cookson, William O ^b   Moffatt, Miriam F ^b   Turner, Paul ^d,e   Parkhill, Julian ^a   Loman, Nicholas J ^c   Walker, Alan W ^a,f  

^a WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

^b NATIONAL HEART AND LUNG INSTITUTE   (United Kingdom)

^c UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^d SHOKLO MALARIA RESEARCH UNIT   (Thailand)

^e UNIVERSITY OF OXFORD   (United Kingdom)

^f ROWETT RESEARCH INSTITUTE   (United Kingdom)

Author keywords

16S rRNA; Contamination; Metagenomics; Microbiome; Microbiota

Indexed keywords

DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; RNA 16S;

DNA CONTAMINATION; DNA SEQUENCE; GENETICS; LABORATORY; METAGENOMICS; MICROFLORA; POLYMERASE CHAIN REACTION; SALMONELLA;

DNA CONTAMINATION; INDICATORS AND REAGENTS; LABORATORIES; METAGENOMICS; MICROBIOTA; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SALMONELLA; SEQUENCE ANALYSIS, DNA;

EID: 84920644670     PISSN: None     EISSN: 17417007     Source Type: Journal    
DOI: 10.1186/s12915-014-0087-z     Document Type: Article

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