Effects of PCR cycle number and DNA polymerase type on the 16S rRNA gene pyrosequencing analysis of bacterial communities

Journal of Microbiology

Volumn 50, Issue 6, 2012, Pages 1071-1074

  Ahn, Jae Hyung ^a   Kim, Byung Yong ^a   Song, Jaekyeong ^a   Weon, Hang Yeon ^a  

^a National Institute of Animal Science   (South Korea)

Author keywords

16S rRNA gene; DNA polymerase; PCR artifacts; PCR cycle number; pyrosequencing

Indexed keywords

RNA 16S; TAQ POLYMERASE;

ARTICLE; BACTERIUM; CLASSIFICATION; GENETICS; HIGH THROUGHPUT SEQUENCING; METHODOLOGY; POLYMERASE CHAIN REACTION;

BACTERIA; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; TAQ POLYMERASE;

BACTERIA (MICROORGANISMS);

EID: 84871751049     PISSN: 12258873     EISSN: 19763794     Source Type: Journal    
DOI: 10.1007/s12275-012-2642-z     Document Type: Article

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