The truth about metagenomics: Quantifying and counteracting bias in 16S rRNA studies Ecological and evolutionary microbiology

BMC Microbiology

Volumn 15, Issue 1, 2015, Pages

  Brooks, J Paul ^a   Edwards, David J ^a   Harwich, Michael D ^a   Rivera, Maria C ^a   Fettweis, Jennifer M ^a   Serrano, Myrna G ^a   Reris, Robert A ^a   Sheth, Nihar U ^a   Huang, Bernice ^a   Girerd, Philippe ^b   Strauss, Jerome F ^b   Jefferson, Kimberly K ^a   Buck, Gregory A ^a  

^a VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

^b VIRGINIA COMMONWEALTH UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Assessments of microbial community structure via metagenomics; DNA extraction bias; Next generation sequencing; PCR bias; Quality control

Indexed keywords

RNA 16S; BACTERIAL DNA;

ANALYTICAL EQUIPMENT; ARTICLE; ATOPOBIUM VAGINAE; BACTERIAL STRAIN; BACTERIUM; BACTERIUM CONTAMINATION; COMMUNITY STRUCTURE; CONTROLLED STUDY; DNA EXTRACTION; DNA EXTRACTION KIT; DNA SEQUENCE; GARDNERELLA VAGINALIS; GENE AMPLIFICATION; GENE DOSAGE; GENOME SIZE; HUMAN; LACTOBACILLUS; LACTOBACILLUS CRISPATUS; LACTOBACILLUS INERS; METAGENOMICS; MICROBIAL COMMUNITY; MICROBIAL DIVERSITY; MICROBIOME; NEXT GENERATION SEQUENCING; NONHUMAN; POLYMERASE CHAIN REACTION; PREVOTELLA BIVIA; QUALITY CONTROL; RNA PROCESSING; SAMPLING BIAS; SAMPLING ERROR; SNEATHIA AMNII; STREPTOCOCCUS AGALACTIAE; TAXONOMIC IDENTIFICATION; VAGINA PH; ARTIFACT; BIOLOGICAL MODEL; CLASSIFICATION; DEVICES; FEMALE; GENETICS; HIGH THROUGHPUT SEQUENCING; ISOLATION AND PURIFICATION; MICROBIAL CONSORTIUM; MICROBIOLOGY; MICROFLORA; PHYLOGENY; PROCEDURES; RNA GENE; SPECIMEN HANDLING; STANDARDS; STATISTICAL BIAS; VAGINA;

BACTERIA (MICROORGANISMS);

ARTIFACTS; BACTERIA; BIAS (EPIDEMIOLOGY); DNA, BACTERIAL; FEMALE; GENES, RRNA; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; METAGENOMICS; MICROBIAL CONSORTIA; MICROBIOTA; MODELS, BIOLOGICAL; PHYLOGENY; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SPECIMEN HANDLING; VAGINA;

EID: 85011974719     PISSN: None     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/s12866-015-0351-6     Document Type: Article

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