The impact of different DNA extraction kits and laboratories upon the assessment of human gut microbiota composition by 16S rRNA gene sequencing

PLoS ONE

Volumn 9, Issue 2, 2014, Pages

  Kennedy, Nicholas A ^a   Walker, Alan W ^b   Berry, Susan H ^c   Duncan, Sylvia H ^d   Farquarson, Freda M ^d   Louis, Petra ^d   Thomson, John M ^e   Satsangi, Jack ^a   Flint, Harry J ^d   Parkhill, Julian ^b   Lees, Charlie W ^a   Hold, Georgina L ^c  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

^c UNIVERSITY OF ABERDEEN   (United Kingdom)

^d ROWETT RESEARCH INSTITUTE   (United Kingdom)

^e ABERDEEN ROYAL INFIRMARY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; BACTERIAL RNA; RNA 16S; DIAGNOSTIC KIT;

ARTICLE; BACTERIAL GENE; BACTEROIDACEAE; CASE REPORT; CLOSTRIDIACEAE; CONTROLLED STUDY; DIAGNOSTIC KIT; DNA EXTRACTION; ENTEROBACTERIACEAE; ERYSIPELOTRICHACEAE; FECES ANALYSIS; GENE SEQUENCE; HUMAN; INFLAMMATORY BOWEL DISEASE; INTESTINE FLORA; LACHNOSPIRACEAE; MICROBIAL COMMUNITY; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PORPHYROMONADACEAE; PYROSEQUENCING; RUMINOCOCCACEAE; DNA SEQUENCE; GASTROINTESTINAL TRACT; GENETICS; LABORATORY; MICROBIOLOGY; MICROFLORA; PROCEDURES;

DNA, BACTERIAL; GASTROINTESTINAL TRACT; HUMANS; LABORATORIES; MICROBIOTA; REAGENT KITS, DIAGNOSTIC; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA;

EID: 84896089558     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0088982     Document Type: Article

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