Effects of polymerase, template dilution and cycle number on PCR based 16 S rRNA diversity analysis using the deep sequencing method

BMC Microbiology

Volumn 10, Issue , 2010, Pages

  Wu, Jin Ya ^a   Jiang, Xiao Tao ^a   Jiang, Yun Xia ^a   Lu, Su Ying ^a   Zou, Fei ^a   Zhou, Hong Wei ^a  

^a SOUTHERN MEDICAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; HYBRID PROTEIN; RNA 16S; BACTERIAL DNA; DNA DIRECTED DNA POLYMERASE; PRIMER DNA; TAQ POLYMERASE;

ARTICLE; ARTIFACT; BACTERIAL GENE; BACTERIUM; COMMUNITY STRUCTURE; DILUTION; DNA TEMPLATE; GENE SEQUENCE; GENETIC VARIABILITY; MICROBIAL COMMUNITY; NONHUMAN; POLYMERASE CHAIN REACTION; SPECIES RICHNESS; BIODIVERSITY; CLASSIFICATION; DNA MICROARRAY; DNA SEQUENCE; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MICROBIOLOGY; NUCLEOTIDE SEQUENCE; RNA GENE; SEDIMENT; SEQUENCE HOMOLOGY; SPECIES DIFFERENCE;

ARTIFACTS; BACTERIA; BASE SEQUENCE; BIODIVERSITY; DNA PRIMERS; DNA, BACTERIAL; DNA-DIRECTED DNA POLYMERASE; GENES, RRNA; GEOLOGIC SEDIMENTS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, NUCLEIC ACID; SOIL MICROBIOLOGY; SPECIES SPECIFICITY; TAQ POLYMERASE; TEMPLATES, GENETIC;

EID: 77957723630     PISSN: 14712180     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/1471-2180-10-255     Document Type: Article

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