Reducing the impact of PCR-mediated recombination in molecular evolution and environmental studies using a new-generation high-fidelity DNA polymerase

BioTechniques

Volumn 47, Issue 4, 2009, Pages 857-866

  Lahr, Daniel J G ^a   Katz, Laura A ^a,b  

^a UNIVERSITY OF MASSACHUSETTS   (United States)

^b SMITH COLLEGE   (United States)

Author keywords

Copy number; Cycle number; Gene family evolution; PCR chimera; Template concentration

Indexed keywords

ACTIN; DNA POLYMERASE; DNA DIRECTED DNA POLYMERASE; RECOMBINANT DNA;

AMOEBA (GENUS); ARCELLA HEMISPHAERICA; ARTICLE; CHIMERA; COMPUTER PROGRAM; DNA ISOLATION; DNA TEMPLATE; ECOLOGICAL GENETICS; GENE AMPLIFICATION; GENE REPLICATION; GENETIC RECOMBINATION; GENETIC VARIABILITY; HAPLOTYPE; MOLECULAR CLONING; MOLECULAR EVOLUTION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; AMOEBA (LIFE CYCLE STAGE); ANIMAL; CHEMISTRY; CLASSIFICATION; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; SEQUENCE HOMOLOGY; TEMPERATURE; TIME;

ARCELLA HEMISPHAERICA;

ACTINS; AMOEBA; ANIMALS; BASE SEQUENCE; CHIMERA; DNA, RECOMBINANT; DNA-DIRECTED DNA POLYMERASE; EVOLUTION, MOLECULAR; GENETIC VARIATION; HAPLOTYPES; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; SEQUENCE HOMOLOGY, NUCLEIC ACID; TEMPERATURE; TEMPLATES, GENETIC; TIME FACTORS;

EID: 75549083380     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000113219     Document Type: Article

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