Single-cell RNA-seq reveals novel regulators of human embryonic stem cell differentiation to definitive endoderm

Genome Biology

Volumn 17, Issue 1, 2016, Pages

  Chu, Li Fang ^a   Leng, Ning ^a,e   Zhang, Jue ^a   Hou, Zhonggang ^a,f   Mamott, Daniel ^a   Vereide, David T ^a   Choi, Jeea ^d   Kendziorski, Christina ^b   Stewart, Ron ^a   Thomson, James A ^a,b,c  

^a Morgridge Institute for Research   (United States)

^b UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d University of Wisconsin Madison   (United States)

^e GENENTECH INC   (United States)

^f HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Brachyury; CRISPR Cas9; Definitive endoderm; Embryonic stem cells; KLF8; Mesendoderm; SCPattern; Single cell RNA seq; Wave Crest

Indexed keywords

CHEMOKINE RECEPTOR CXCR4; ENHANCED GREEN FLUORESCENT PROTEIN; TRANSCRIPTOME; RNA;

ARTICLE; CELL DIFFERENTIATION; CELL FATE; CELL HYPOXIA; CELL LINEAGE; CONTROLLED STUDY; CRISPR CAS SYSTEM; EMBRYO; EMBRYONIC STEM CELL; ENDODERM; GAIN OF FUNCTION MUTATION; GENE; GENE EXPRESSION REGULATION; HUMAN; HUMAN CELL; KLF8 GENE; LOSS OF FUNCTION MUTATION; MOLECULAR MODEL; PHASE TRANSITION; PLURIPOTENT STEM CELL; RNA SEQUENCE; SCREENING TEST; SINGLE CELL ANALYSIS; TRANSCRIPTOMICS; VALIDATION PROCESS; CELL CULTURE TECHNIQUE; CYTOLOGY; GENETICS; GROWTH, DEVELOPMENT AND AGING; HIGH THROUGHPUT SEQUENCING; HUMAN EMBRYONIC STEM CELL; LIVER CELL; METABOLISM; PROCEDURES;

CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; ENDODERM; GENE EXPRESSION REGULATION, DEVELOPMENTAL; HEPATOCYTES; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMAN EMBRYONIC STEM CELLS; HUMANS; PLURIPOTENT STEM CELLS; RNA; SINGLE-CELL ANALYSIS;

EID: 84982085158     PISSN: 14747596     EISSN: 1474760X     Source Type: Journal    
DOI: 10.1186/s13059-016-1033-x     Document Type: Article

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