Human induced pluripotent stem cells free of vector and transgene sequences

Science

Volumn 324, Issue 5928, 2009, Pages 797-801

  Junying, Yu ^a,b,c   Kejin, Hu ^c   Kim, Smuga Otto ^a,b,c   Shulan, Tian ^a,b   Stewart, Ron ^a,b   Slukvin, Igor I ^c,d   Thomson, James A ^a,b,c,d  

^a MORGRIDGE INSTITUTE FOR RESEARCH   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

^c UNIVERSITY OF WISCONSIN   (United States)

^d UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOVIRUS VECTOR; DNA; LENTIVIRUS VECTOR; VIRUS VECTOR; EBV ENCODED NUCLEAR ANTIGEN 1; EBV-ENCODED NUCLEAR ANTIGEN 1; EPSTEIN BARR VIRUS ANTIGEN; TRANSCRIPTION FACTOR;

CELL ORGANELLE; DRUG DEVELOPMENT; GENOME; MUTATION; TRANSPLANTATION;

ADULT; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CELL CLONE; CELL DIFFERENTIATION; CONTROLLED STUDY; EMBRYO; EMBRYONIC STEM CELL; EPSTEIN BARR VIRUS; GENE EXPRESSION; GENE MUTATION; GENE SEQUENCE; GENETIC TRANSDUCTION; HUMAN; HUMAN CELL; MOUSE; NEWBORN; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PLURIPOTENT STEM CELL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SKIN FIBROBLAST; STEM CELL; TRANSGENE; CELL SHAPE; CYTOLOGY; FIBROBLAST; GENE EXPRESSION PROFILING; GENE VECTOR; GENETIC TRANSFECTION; GENETICS; METABOLISM; NUCLEAR REPROGRAMMING; TRANSPLANTATION;

IPS;

CELL DIFFERENTIATION; CELL SHAPE; CLONE CELLS; EMBRYONIC STEM CELLS; EPSTEIN-BARR VIRUS NUCLEAR ANTIGENS; FIBROBLASTS; GENE EXPRESSION PROFILING; GENETIC VECTORS; HUMANS; NUCLEAR REPROGRAMMING; PLASMIDS; PLURIPOTENT STEM CELLS; TRANSCRIPTION FACTORS; TRANSFECTION; TRANSGENES;

EID: 65649116572     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1172482     Document Type: Article

References (23)

1. J. A. Thomson et al., Science 282, 1145 (1998).
2. J. Yu et al., Science 318, 1917 (2007).
3. K. Takahashi et al., Cell 131, 861 (2007).
4. K. Takahashi, S. Yamanaka, Cell 126, 663 (2006).
5. K. Okita, T. Ichisaka, S. Yamanaka, Nature 448, 313 (2007).
6. M. Stadtfeld, M. Nagaya, J. Utikal, G. Weir, K. Hochedlinger, Science 322, 945 (2008).
7. K. Okita, M. Nakagawa, H. Hyenjong, T. Ichisaka, S. Yamanaka, Science 322, 949 (2008).
8. K. Kaji et al., Nature 458, 771 (2009).
9. F. Soldner et al., Cell 136, 964 (2009).
10. K. Woltjen et al., Nature, 10.1038/nature07863 (2009).
11. J. Yates, N. Warren, D. Reisman, B. Sugden, Proc. Natl. Acad. Sci. U.S.A. 81, 3806 (1984).
12. J. L. Yates, N. Warren, B. Sugden, Nature 313, 812 (1985).
13. E. R. Leight, B. Sugden, Mol. Cell. Biol. 21, 4149 (2001).
14. J. L. Yates, N. Guan, J. Virol. 65, 483 (1991).
15. A. Nanbo, A. Sugden, B. Sugden, EMBO J. 26, 4252 (2007).
16. A. D. Ebert et al., Nature 457, 277 (2009).
17. J. Liao et al., Cell Res. 18, 600 (2008).
18. G. I. Evan et al., Cell 69, 119 (1992).
19. W. C. Hahn et al., Nature 400, 464 (1999).
20. T. Aasen et al., Nat. Biotechnol. 26, 1276 (2008).
21. Y. Shi et al., Cell Stem Cell 3, 568 (2008).
22. D. Huangfu et al., Nat. Biotechnol. 26, 795 (2008).
23. Funding was provided by the Charlotte Geyer Foundation and NIH (grants P51 RR000167 and P01 GM081629). We thank A. Griep, P. Powers, B. Sugden, M. Probasco, A. Elwell, C. Glennon, V. Ruotti, and D. J. Faupel for assistance and advice. The authors declare competing financial interests: J.A.T. is a founder, stock owner, consultant, and board member of Cellular Dynamics International (CDI). He also serves as a scientific adviser to and has financial interests in Tactics II Stem Cell Ventures. I.I.S. is a founder, stock owner, and consultan for CDI. The authors are filing a patent based on the results reported in this paper. Combination 6 and 19 episomal vectors are deposited in Addgene (Cambridge, MA), and vector-free human iPS cell subclones are deposited in the WiCell International Stem Cell (WISC) Bank (Madison, WI). Microarray data are deposited in the Gene Expression Omnibus (GEO) database (accession number GSE15148).