Unfolding the mechanism of the AAA+ unfoldase VAT by a combined cryo-EM, solution NMR study

Proceedings of the National Academy of Sciences of the United States of America

Volumn 113, Issue 29, 2016, Pages E4090-W4199

  Huang, Rui ^a,b   Ripstein, Zev A ^a,c   Augustyniak, Rafal ^a,b   Lazniewski, Michal ^d,e   Ginalski, Krzysztof ^d   Kay, Lewis E ^a,b,c   Rubinstein, John L ^a,c  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c HOSPITAL FOR SICK CHILDREN RESEARCH INSTITUTE   (Canada)

^d UNIVERSITY OF WARSAW   (Poland)

^e MEDICAL UNIVERSITY OF WARSAW   (Poland)

Author keywords

Cryo EM; Methyl trosy NMR; Proteasome; Protein dynamics; VAT

Indexed keywords

1,10 PHENANTHROLINE COPPER; ADENOSINE TRIPHOSPHATASE; MALEIMIDE; NUCLEOTIDE; PROTEASOME; VALOSIN CONTAINING PROTEIN; ARCHAEAL PROTEIN; VAT PROTEIN, THERMOPLASMA ACIDOPHILUM;

CLASSIFICATION ALGORITHM; CONFERENCE PAPER; CONFORMATIONAL TRANSITION; CROSS LINKING; CRYOELECTRON MICROSCOPY; CRYSTAL STRUCTURE; DIFFUSION COEFFICIENT; ENZYME ISOLATION; FLUORESCENCE; FREEZING; HYDROLYSIS; NATIVE POLYACRYLAMIDE GEL ELECTROPHORESIS; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN SECONDARY STRUCTURE; PROTEIN UNFOLDING; SIZE EXCLUSION CHROMATOGRAPHY; THERMOPLASMA ACIDOPHILUM; X RAY CRYSTALLOGRAPHY; CHEMISTRY; ENZYMOLOGY; GENETICS; NUCLEAR MAGNETIC RESONANCE; THERMOPLASMA;

ARCHAEAL PROTEINS; CRYOELECTRON MICROSCOPY; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN CONFORMATION; THERMOPLASMA; VALOSIN CONTAINING PROTEIN;

EID: 84978842964     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1603980113     Document Type: Article

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