Description and comparison of algorithms for correcting anisotropic magnification in cryo-EM images

Journal of Structural Biology

Volumn 192, Issue 2, 2015, Pages 209-215

  Zhao, Jianhua ^a,b   Brubaker, Marcus A ^c   Benlekbir, Samir ^a   Rubinstein, John L ^a,b,c  

^a HOSPITAL FOR SICK CHILDREN   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c UNIVERSITY OF TORONTO   (Canada)

Author keywords

Anisotropic magnification; Contrast transfer function; Correction; Cryo EM; Differential magnification; Direct detector device; Direct electron detector; Distortion

Indexed keywords

ALGORITHM; ANISOTROPIC MAGNIFICATION; ANISOTROPY; ARTICLE; ARTIFACT; CAMERA; CONTRAST TRANSFER FUNCTION; CONTROLLED STUDY; CRYOELECTRON MICROSCOPY; DIRECT DETECTOR DEVICE CAMERA; FOURIER TRANSFORMATION; ILLUMINATION; IMAGE ANALYSIS; IMAGE DISPLAY; IMAGE PROCESSING; IMAGE QUALITY; IMAGING AND DISPLAY; MICROSCOPE IMAGE; OPTICAL RESOLUTION; PRIORITY JOURNAL; DEVICES; IMAGE ENHANCEMENT; PROCEDURES; THREE DIMENSIONAL IMAGING; ULTRASTRUCTURE;

MULTIPROTEIN COMPLEX; PROTEIN; THALLIUM; THALLIUM CHLORIDE;

ALGORITHMS; ANISOTROPY; CRYOELECTRON MICROSCOPY; IMAGE ENHANCEMENT; IMAGING, THREE-DIMENSIONAL; MULTIPROTEIN COMPLEXES; PROTEINS; THALLIUM;

EID: 84946486192     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2015.06.014     Document Type: Article

References (10)

1. Baldwin J., Henderson R. Measurement and evaluation of electron diffraction patterns from two-dimensional crystals. Ultramicroscopy 1984, 14(4):319-335.
2. Bracewell R.N., Bracewell R. The Fourier Transform and Its Applications 1986, vol. 31999. McGraw-Hill, New York.
3. Grant T., Grigorieff N. Measuring the optimal exposure for single particle cryo-EM using a 2.6 å reconstruction of rotavirus vp6. eLife 2015, e06980.
4. Kühlbrandt W. The resolution revolution. Science 2014, 343(6178):1443-1444.
5. Li X., Mooney P., Zheng S., Booth C.R., Braunfeld M.B., Gubbens S., Agard D.A., Cheng Y. Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM. Nat. Methods 2013, 10(6):584-590.
6. Mindell J.A., Grigorieff N. Accurate determination of local defocus and specimen tilt in electron microscopy. J. Struct. Biol. 2003, 142(3):334-347.
7. Scheres S.H. Relion: implementation of a bayesian approach to cryo-EM structure determination. J. Struct. Biol. 2012, 180(3):519-530.
8. Smith M.T., Rubinstein J.L. Beyond blob-ology. Science 2014, 345(6197):617-619.
9. Toyoshima C., Unwin N. Contrast transfer for frozen-hydrated specimens: determination from pairs of defocused images. Ultramicroscopy 1988, 25(4):279-291.
10. Wade R. A brief look at imaging and contrast transfer. Ultramicroscopy 1992, 46(1):145-156.