Discovery of high affinity anti-ricin antibodies by B cell receptor sequencing and by yeast display of combinatorial VH:VL libraries from immunized animals

mAbs

Volumn 8, Issue 6, 2016, Pages 1035-1044

  Wang, Bo ^a   Lee, Chang Han ^a   Johnson, Erik L ^a   Kluwe, Christien A ^b   Cunningham, Josephine C ^b   Tanno, Hidetaka ^a   Crooks, Richard M ^b   Georgiou, George ^a,b   Ellington, Andrew D ^b  

^a The University of Texas at Austin   (United States)

^b UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

Antibody repertoire analysis; next generation sequencing; ricin A chain; yeast surface display

Indexed keywords

B LYMPHOCYTE RECEPTOR; RICIN ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; ANTIBODY; LYMPHOCYTE ANTIGEN RECEPTOR; MESSENGER RNA; NANOBODY; RICIN;

AMINO ACID SUBSTITUTION; BINDING AFFINITY; CELL SELECTION; CELL SURFACE DISPLAY; COMBINATORIAL LIBRARY; CONFORMATIONAL TRANSITION; EC50; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE SEQUENCE; HIGH THROUGHPUT SEQUENCING; IMMUNE RESPONSE; IMMUNIZATION; NEXT GENERATION SEQUENCING; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; RNA EXTRACTION; SEQUENCE ANALYSIS; SURFACE PLASMON RESONANCE; YEAST SURFACE DISPLAY; ANIMAL; ANTIBODY AFFINITY; B LYMPHOCYTE; BAGG ALBINO MOUSE; BONE MARROW CELL; CHEMISTRY; CYTOLOGY; FEMALE; GENETICS; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; LYMPH NODE; MOUSE; PEPTIDE LIBRARY; PROCEDURES; SACCHAROMYCES CEREVISIAE; SPLEEN;

ANIMALS; ANTIBODIES; ANTIBODY AFFINITY; B-LYMPHOCYTES; BONE MARROW CELLS; CELL SURFACE DISPLAY TECHNIQUES; FEMALE; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; IMMUNIZATION; LYMPH NODES; MICE; MICE, INBRED BALB C; PEPTIDE LIBRARY; RECEPTORS, ANTIGEN, B-CELL; RICIN; RNA, MESSENGER; SACCHAROMYCES CEREVISIAE; SINGLE-DOMAIN ANTIBODIES; SPLEEN;

EID: 84976329717     PISSN: 19420862     EISSN: 19420870     Source Type: Journal    
DOI: 10.1080/19420862.2016.1190059     Document Type: Article

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