Monoclonal antibodies isolated without screening by analyzing the variable-gene repertoire of plasma cells

Nature Biotechnology

Volumn 28, Issue 9, 2010, Pages 965-969

  Reddy, Sai T ^a   Ge, Xin ^a   Miklos, Aleksandr E ^b   Hughes, Randall A ^b   Kang, Seung Hyun ^a   Hoi, Kam Hon ^a   Chrysostomou, Constantine ^a   Hunicke Smith, Scott P ^b   Iverson, Brent L ^b   Tucker, Philip W ^b   Ellington, Andrew D ^b   Georgiou, George ^a,b  

^a The University of Texas at Austin   (United States)

^b UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY FRAGMENT; B CELLS; BINDING AFFINITIES; BIOINFORMATIC ANALYSIS; BONE MARROW; DNA SEQUENCING; GENE SYNTHESIS; HIGH-THROUGHPUT; HIGH-THROUGHPUT SCREENING; MAMMALIAN CELLS; MONOCLONAL ANTIBODIES (MABS); NANOMOLAR; PLASMA CELLS; RECOMBINANT ANTIBODY; RELATIVE FREQUENCIES;

BINDING ENERGY; BIOINFORMATICS; GENE ENCODING; IMMUNIZATION; MAMMALS; MONOCLONAL ANTIBODIES; SURFACE PLASMON RESONANCE;

ANTIGENS;

COMPLEMENTARY DNA; IMMUNOGLOBULIN G ANTIBODY; MONOCLONAL ANTIBODY; RECOMBINANT ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANIMAL CELL; ANIMAL EXPERIMENT; ANTIBODY DETECTION; ANTIBODY ISOLATION; ANTIBODY SCREENING; ANTIBODY TITER; ANTIGEN SPECIFICITY; ARTICLE; B LYMPHOCYTE; BINDING AFFINITY; BIOINFORMATICS; BONE MARROW CELL; CELL IMMORTALIZATION; CELL STRAIN HEK293; CONTROLLED STUDY; DNA SEQUENCE; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; GENE SEQUENCE; GENE SYNTHESIS; GENETIC VARIABILITY; HIGH THROUGHPUT SCREENING; IMMUNIZATION; MOUSE; NONHUMAN; OPEN READING FRAME; PLASMA CELL; POLARIZATION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SURFACE PLASMON RESONANCE;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIGENS; BASE SEQUENCE; COMPLEMENTARITY DETERMINING REGIONS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; IMMUNIZATION; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN VARIABLE REGION; MICE; MOLECULAR SEQUENCE DATA; PLASMA CELLS;

MAMMALIA; MUS;

EID: 77956688921     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.1673     Document Type: Article

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