Delivery of Cas9 Protein into Mouse Zygotes through a Series of Electroporation Dramatically Increases the Efficiency of Model Creation

Journal of Genetics and Genomics

Volumn 43, Issue 5, 2016, Pages 319-327

  Wang, Wenbo ^a   Kutny, Peter M ^a   Byers, Shannon L ^a   Longstaff, Charles J ^a   DaCosta, Michael J ^a   Pang, Changhong ^a   Zhang, Yingfan ^a   Taft, Robert A ^a   Buaas, Frank W ^a   Wang, Haoyi ^a,b  

^a JACKSON LABORATORY   (United States)

^b INSTITUTE OF ZOOLOGY   (China)

Author keywords

CRISPR Cas9; Electroporation; Mouse zygote

Indexed keywords

CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN; DNA FRAGMENT; MESSENGER RNA; PROTEIN CAS9; UNCLASSIFIED DRUG; DEOXYRIBONUCLEASE I;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CRISPR CAS SYSTEM; DNA MODIFICATION; ELECTROPORATION; GENE DELIVERY SYSTEM; GENE LOCUS; GENE TECHNOLOGY; GENOMIC FRAGMENT; INDEL MUTATION; MOUSE; MOUSE MODEL; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; ZYGOTE; ZYGOTE ELECTROPORATION OF NUCLEASE TECHNOLOGY; ANIMAL; GENETICS; METABOLISM; PROTEIN TRANSPORT;

ANIMALS; DEOXYRIBONUCLEASE I; ELECTROPORATION; MICE; PROTEIN TRANSPORT; RNA, MESSENGER; ZYGOTE;

EID: 84975512687     PISSN: 16738527     EISSN: 18735533     Source Type: Journal    
DOI: 10.1016/j.jgg.2016.02.004     Document Type: Article

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