Efficient Gene Targeting in Zebrafish Mediated by a Zebrafish-Codon-Optimized Cas9 and Evaluation of Off-Targeting Effect

Journal of Genetics and Genomics

Volumn 41, Issue 1, 2014, Pages 43-46

  Liu, Da ^a,b   Wang, Zhanxiang ^a   Xiao, An ^a   Zhang, Yutian ^a   Li, Wenyuan ^a   Zu, Yao ^a,c   Yao, Shaohua ^c   Lin, Shuo ^b,c   Zhang, Bo ^a  

^a Peking University   (China)

^b PEKING UNIVERSITY   (China)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; FUMARATE HYDRATASE; MESSENGER RNA; RIBOSOMAL PROTEIN L11; RIBOSOME PROTEIN; TRYPTOPHAN HYDROXYLASE; TRYPTOPHAN HYDROXYLASE 1A; UNCLASSIFIED DRUG;

BASE MISPAIRING; CAS9 GENE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CODON; CRISPR CAS SYSTEM; DNA MODIFICATION; EMBRYO; EVALUATION STUDY; GENE; GENE MUTATION; GENE TARGETING; IN VITRO STUDY; INDEL MUTATION; LETTER; LOSS OF FUNCTION MUTATION; MUTAGENESIS; MUTAGENIC ACTIVITY; MUTATION RATE; NONHUMAN; PHENOTYPE; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; WOLF HIRSCHHORN SYNDROME; ZEBRA FISH;

DANIO RERIO;

ANIMALS; BASE SEQUENCE; CODON; CRISPR-CAS SYSTEMS; FISH PROTEINS; GENE TARGETING; MOLECULAR SEQUENCE DATA; ZEBRAFISH;

EID: 84893651502     PISSN: 16738527     EISSN: 18735533     Source Type: Journal    
DOI: 10.1016/j.jgg.2013.11.004     Document Type: Letter

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