Efficient CRISPR/cas9-mediated genome editing in mice by zygote electroporation of nuclease

Genetics

Volumn 200, Issue 2, 2015, Pages 423-430

  Qin, Wenning ^a   Dion, Stephanie L ^a   Kutny, Peter M ^a   Zhang, Yingfan ^a   Cheng, Albert W ^a   Jillette, Nathaniel L ^a   Malhotra, Ankit ^a   Geurts, Aron M ^b   Chen, Yi Guang ^b   Wang, Haoyi ^a,c  

^a JACKSON LABORATORY   (United States)

^b MEDICAL COLLEGE OF WISCONSIN   (United States)

^c INSTITUTE OF ZOOLOGY   (China)

Author keywords

Cas9; CRISPR; Electroporation; Mouse zygote

Indexed keywords

GUIDE RNA; MESSENGER RNA; NUCLEASE; OLIGONUCLEOTIDE; CRISPR-CAS SYSTEM GUIDE RNA; ENDONUCLEASE;

ADAPTIVE IMMUNITY; ALLELE; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BLASTOCYST; CONTROLLED STUDY; CRISPR CAS SYSTEM; DNA END JOINING REPAIR; DNA SEQUENCE; ELECTROPORATION; EMBRYO; EMBRYO TRANSFER; FEMALE; GENETIC ENGINEERING; GENOME; GENOME EDITING; IN VITRO STUDY; INDEL MUTATION; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; WILD TYPE; ZONA PELLUCIDA; ZYGOTE; ZYGOTE ELECTROPORATION NUCLEASE; ANIMAL; GENE LOCUS; GENE TARGETING; GENETICS; GENOMICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; RNA EDITING; SEQUENCE ALIGNMENT;

MUS;

ANIMALS; BASE SEQUENCE; CRISPR-CAS SYSTEMS; ELECTROPORATION; ENDONUCLEASES; FEMALE; GENE TARGETING; GENETIC LOCI; GENOME; GENOMICS; INDEL MUTATION; MICE; MOLECULAR SEQUENCE DATA; RNA EDITING; RNA, GUIDE, CRISPR-CAS SYSTEMS; RNA, MESSENGER; SEQUENCE ALIGNMENT; ZYGOTE;

EID: 84931385060     PISSN: 00166731     EISSN: 19432631     Source Type: Journal    
DOI: 10.1534/genetics.115.176594     Document Type: Article

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