CRISPR-STAT: An easy and reliable PCR-based method to evaluate target-specific sgRNA activity

Nucleic Acids Research

Volumn 43, Issue 22, 2015, Pages

  Carrington, Blake ^a   Varshney, Gaurav K ^a   Burgess, Shawn M ^a   Sood, Raman ^a  

^a NATIONAL HUMAN GENOME RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CRISPR ASSOCIATED PROTEIN; MESSENGER RNA; RNA; SINGLE GUIDE RNA; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; CAPILLARY ELECTROPHORESIS; CONTROLLED STUDY; EMBRYO; ENZYME ACTIVITY; GENE; GENE SEQUENCE; GENE TARGETING; GERMLINE MUTATION; HIGH RESOLUTION MELTING ANALYSIS; INDEL MUTATION; LOXHD1B GENE; MAN2B1 GENE; MARVELD2B GENE; MOLECULAR CLONING; MUTAGENESIS; MYH9A GENE; MYO15AA GENE; MYO6A GENE; NONHUMAN; POLYMERASE CHAIN REACTION; PRDX3 GENE; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; REVIEW; RNA CLEAVAGE; RNA EDITING; RNA SYNTHESIS; SENSITIVITY AND SPECIFICITY; SH3PXD2AA GENE; SOMATIC EMBRYOGENESIS; SOMATIC TISSUE ACTIVITY TEST; ZEBRA FISH; ANIMAL; CRISPR CAS SYSTEM; FLUORESCENCE; GENETICS; METABOLISM; PROCEDURES; VALIDATION STUDY;

ANIMALS; CRISPR-CAS SYSTEMS; FLUORESCENCE; INDEL MUTATION; POLYMERASE CHAIN REACTION; RNA; ZEBRAFISH;

EID: 84964337221     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkv802     Document Type: Review

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