A PCR based protocol for detecting indel mutations induced by TALENs and CRISPR/Cas9 in zebrafish

PLoS ONE

Volumn 9, Issue 6, 2014, Pages

  Yu, Chuan ^a   Zhang, Yaguang ^a   Yao, Shaohua ^a   Wei, Yuquan ^a  

^a SICHUAN UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CRISPR ASSOCIATED PROTEIN; CRISPR ASSOCIATED SYSTEM 9; DNA FRAGMENT; GENOMIC DNA; MESSENGER RNA; MICRORNA; NUCLEASE; TRANSCRIPTION ACTIVATOR LIKE EFFECTER NUCLEASE; UNCLASSIFIED DRUG;

ADULT; AMPLICON; ARTICLE; BIOASSAY; COMPARATIVE STUDY; CONTROLLED STUDY; DNA FLANKING REGION; DNA SEQUENCE; DOUBLE STRANDED DNA BREAK; EMBRYO; FEMALE; GENE; GENE AMPLIFICATION; GENE FREQUENCY; GENE LOCUS; GENOMICS; INDEL MUTATION; MALE; MOLECULAR CLONING; MUTATIONAL ANALYSIS; NONHUMAN; NSD2 GENE; NSD3 GENE; POLYMERASE CHAIN REACTION; PROMOTER REGION; QUANTITATIVE ANALYSIS; RESTRICTION SITE; T VECTOR BASED MUTANT COLONY IDENTIFICATION ASSAY; WILD TYPE; ZEBRA FISH; ANIMAL; CRISPR CAS SYSTEM; GENETICS; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; SEQUENCE ALIGNMENT;

ANIMALS; BASE SEQUENCE; CRISPR-CAS SYSTEMS; DNA MUTATIONAL ANALYSIS; FEMALE; GENETIC LOCI; INDEL MUTATION; MALE; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; ZEBRAFISH;

EID: 84902440389     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0098282     Document Type: Article

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