A high-throughput screening strategy for detecting CRISPR-Cas9 induced mutations using next-generation sequencing

BMC Genomics

Volumn 15, Issue 1, 2014, Pages

  Bell, Charles C ^a   Magor, Graham W ^a   Gillinder, Kevin R ^a   Perkins, Andrew C ^a,b  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b PRINCESS ALEXANDRA HOSPITAL   (Australia)

Author keywords

Cas9; CRISPR; CRISPR Cas9; Genome editing; Indel; Next generation sequencing; Screening strategy

Indexed keywords

ANIMAL CELL; ARTICLE; CLONE; CONTROLLED STUDY; CRISPR CAS SYSTEM; EMBRYO; EMBRYONIC STEM CELL; EVX1 GENE; GENE MUTATION; GENETIC TRANSFECTION; HETEROZYGOTE; HIGH THROUGHPUT SCREENING; HOMEOBOX; HOMOZYGOTE; MOUSE; MUTANT; NEXT GENERATION SEQUENCING; NONHUMAN; PROCESS DEVELOPMENT; STEM CELL CULTURE; ANIMAL; CELL CLONE; GENETICS; HIGH THROUGHPUT SEQUENCING; METABOLISM; MUTATION; PROCEDURES;

EUKARYOTA;

EVX1 PROTEIN, MOUSE; HOMEODOMAIN PROTEIN;

ANIMALS; CLONE CELLS; CRISPR-CAS SYSTEMS; EMBRYONIC STEM CELLS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HOMEODOMAIN PROTEINS; MICE; MUTATION;

EID: 84924287971     PISSN: None     EISSN: 14712164     Source Type: Journal    
DOI: 10.1186/1471-2164-15-1002     Document Type: Article

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