Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris

Journal of Biotechnology

Volumn 235, Issue , 2016, Pages 139-149

  Weninger, Astrid ^a   Hatzl, Anna Maria ^a   Schmid, Christian ^a   Vogl, Thomas ^a   Glieder, Anton ^a  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

CRISPR Cas9; Gene replacement by homologous recombination; Genome engineering; Multiplexing; Pichia pastoris; Synthetic biology

Indexed keywords

COMBINATORIAL OPTIMIZATION; DNA SEQUENCES; MACHINERY; METABOLIC ENGINEERING; MULTIPLEXING; NUCLEIC ACIDS; POLYMERS; PRECISION ENGINEERING; RNA; YEAST;

CRISPR/CAS9; GENOME ENGINEERING; HOMOLOGOUS RECOMBINATION; PICHIA PASTORIS; SYNTHETIC BIOLOGY;

GENES;

DNA DIRECTED RNA POLYMERASE III; RNA POLYMERASE II;

CARBON SOURCE; CODON; CRISPR CAS SYSTEM; DNA INTEGRATION; DNA MODIFICATION; DNA SEQUENCE; GENE DELETION; GENE DISRUPTION; GENE EXPRESSION; GENETIC ENGINEERING; HOMOLOGOUS RECOMBINATION; KOMAGATAELLA PASTORIS; METABOLIC ENGINEERING; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; REVIEW; SACCHAROMYCES CEREVISIAE; PICHIA; SYNTHETIC BIOLOGY;

CRISPR-CAS SYSTEMS; GENETIC ENGINEERING; PICHIA; SYNTHETIC BIOLOGY;

EID: 84963593243     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2016.03.027     Document Type: Review

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