Cloning and disruption of the PpURA5 gene and construction of a set of integration vectors for the stable genetic modification of Pichia pastoris

Yeast

Volumn 20, Issue 15, 2003, Pages 1279-1290

  Nett, Juergen H ^a   Gerngross, Tillman U ^b  

^a GlycoFi Incorporated   (United States)

^b DARTMOUTH COLLEGE   (United States)

Author keywords

OCH1; Orotate phosphoribosyltransferase; Pichia pastoris; SEC65; Stable genetic modification; URA3 blaster; URA5; URA5 blaster

Indexed keywords

BETA GALACTOSIDASE; KANAMYCIN; OROTATE PHOSPHORIBOSYLTRANSFERASE; PLASMID VECTOR;

ARTICLE; AUXOTROPHY; CONTROLLED STUDY; DNA MODIFICATION; GENE AMPLIFICATION; GENE CASSETTE; GENE DISRUPTION; GENE IDENTIFICATION; GENE SEQUENCE; GENETIC STABILITY; GENETIC TRANSFORMATION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PICHIA PASTORIS; PRIORITY JOURNAL; SEQUENCE ANALYSIS; WILD TYPE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DNA, FUNGAL; DRUG RESISTANCE, FUNGAL; FUNGAL PROTEINS; GENETIC VECTORS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; OROTATE PHOSPHORIBOSYLTRANSFERASE; OROTIC ACID; PICHIA; PLASMIDS; POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; TRANSFORMATION, GENETIC;

PICHIA; PICHIA PASTORIS;

EID: 0344466719     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/yea.1049     Document Type: Article

References (35)

1. Alani E, Cao L, Kleckner N. 1987. A method for gene disruption that allows repeated use of the URA3 selection in the construction of multiply disrupted yeast strains. Genetics 116: 541-545.
2. Bai X, Larsen M, Meinhardt F. 1999. The URA5 gene encoding orotate-phosphoribosyl transferase of the yeast Kluyveromyces lactis: cloning sequencing and use as a selectable marker. Yeast 15: 1393-1398.
3. Bergès T, Barreau C. 1991. Isolation of uridine auxotrophs from Trichoderma reesei and efficient transformation with the cloned ura3 and ura5 genes. Curr Genet 19: 359-365.
4. Boehm T, Pirie-Shepherd S, Trinh L-B, Shiloach J, Folkman J. 1999. Disruption of the KEX1 gene in Pichia pastoris allows expression of full-length murine and human endostatin. Yeast 15: 563-572.
5. Boeke MD, LaCroute F, Fink GR. 1984. A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol Gen Genet 197: 345-346.
6. Bretthauer RK, Castellino FJ. 1999. Glycosylation of Pichia pastoris-derived proteins. Biotechnol Appl Biochem 30: 193-200.
7. Brooke JB, Hammond AT, Reinke CA, Glick BS. 2002. De novo formation of transitional ER sites and Golgi structures in Pichia pastoris. Nature Cell Biol 4: 750-756.
8. 2) sugar chains in Saccharomyces cerevisiae. J Biol Chem 273: 26 298-26 304.
9. Choi B-K, Bobrowicz P, Davidson RC, et al. 2003. Use of combinatorial genetic libraries to humanize N-linked glycosylation in the yeast Pichia pastoris. Proc Natl Acad Sci USA 100: 5022-5027.
10. Cregg JM, Barringer KJ, Hessler AY, Madden KR. 1985. Pichia pastoris as a host system for transformations. Mol Cell Biol 5: 3376-3385.
11. De Montigny J, Belarbi A, Hubert J-C, Lacroute F. 1989. Structure and expression of the URA5 gene of Saccharomyces cerevisiae. Mol Gen Genet 215: 455-462.
12. De Montigny J, Kern L, Hubert J-C, Lacroute F. 1990. Cloning and sequencing of URA10, a second gene encoding orotate phosphoribosyl transferase in Saccaromyces cerevisiae. Curr Genet 17: 105-111.
13. Edman JC, Kwon-Chung KJ. 1990. Isolation of the URA5 gene from Cryptococcus neoformans var. neoformans and its use as a selective marker for transformation. Mol Cell Biol 10: 4538-4544.
14. Gould SJ, McCollum D, Spong AP, Heyman JA, Subramani S. 1992. Development of the yeast Pichia pastoris as a model organism for a genetic and molecular analysis of peroxisome assembly. Yeast 8: 613-628.
15. Güldener U, Heck S, Fiedler T, Beinhauer J, Hegemann JH. 1996. A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Res 24: 2519-2524.
16. Higgins DR, Busser K, Cominsky J, et al. 1998. Small vectors for expression based on dominant drug resistance with direct multicopy selection. Methods Mol Biol 103: 41-53.
17. Higgins DR, Cregg JM. 1998. Introduction to Pichia pastoris. Methods Mol Biol 103: 1-15.
18. Hirose M, Kameyama S, Ohi H. 2002. Characterization of N-linked oligosaccharides attached to recombinant human antithrombin expressed in the yeast Pichia pastoris. Yeast 19: 1191-1202.
19. Kimura M, Kamakura T, Tao QZ, Kaneko I, Yamaguchi I. 1994. Cloning of the blasticidin S deaminase gene BSD from Aspergillus terreus and its use as a selectable marker for Schizosaccharomyces pombe and Pyricularia oryzae. Mol Gen Genet 242: 121-129.
20. Lin Cereghino GP, Cregg JM. 1999. Applications of yeast in biotechnology: protein production and genetic analysis. Curr Opin Biotechnol 10: 422-427.
21. Lin Cereghino J, Cregg JM. 2000. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 24: 45-66.
22. Lin Cereghino GP, Lin Cereghino J, Sunga AJ, et al. 2001. New selectable marker/auxotrophic host strain combinations for molecular genetic manipulation of Pichia pastoris. Gene 263: 159-169.
23. Lu P, Davis BP, Hendrick J, Jeffries TW. 1998. Cloning and disruption of the β-isopropylmalate dehydrogenase gene (Leu2) of Pichia stipidis with URA3 and recovery of the double auxotroph. Appl Microbiol Biotechnol 49: 141-146.
24. Ménard Szczebara F, Chandelier C, Villeret C, et al. 2003. Total biosynthesis of hydrocortisone from a simple carbon source in yeast. Nature Biotechnol 21: 143-149.
25. Montesino R, García R, Quintero O, Cremata JA. 1998. Variation in N-linked oligosaccharide structures on heterologous proteins secreted by the methylotropic yeast Pichia pastoris. Protein Expr Purif 14: 197-207.
26. Rose TM, Schultz ER, Henikoff JG, et al. 1998. Consensus-degenerate hybrid oligonucleotide primers for amplification of distantly related sequences. Nucleic Acids Res 26: 1628-1635.
27. Rossanese OW, Sonderholm J, Bevis BJ, et al. 1999. Golgi structure correlates with transitional endoplasmic reticulum organization in Pichia pastoris and Saccharomyces cerevisiae. J Cell Biol 145: 69-81.
28. Sakai Y, Koller A, Rangell LK, Keller GA, Subramani S. 1998. Peroxisome degradation by microautophagy in Pichia pastoris: identification of specific steps and morphological intermediates. J Cell Biol 141: 625-636.
29. Sánchez M, Prado M, Iglesias FJ, Domínguez A. 1995. Cloning and sequencing of the URA5 gene from the yeast Yarrowia lipolytica. Yeast 11: 425-433.
30. Sánchez M, Domínguez A. 2001. Gene order in a 10 275 bp fragment of Yarrowia lipolytica, including adjacent YlURA5 and YlSEC65 genes conserved in four species. Yeast 18: 807-813.
31. Sears IB, O'Connor J, Rossanese OW, Glick BS. 1998. A versatile set of vectors for constitutive and regulated gene expression in Pichia pastoris. Yeast 14: 783-790.
32. Soderholm J, Bevis BJ, Glick BS. 2001. Vector for pop-in/pop-out gene replacement in Pichia pastoris. Biotechniques 31: 306-312.
33. Tuttle DL, Dunn WA Jr. 1995. Divergent modes of autophagy in the methylotropic yeast Pichia pastoris. J Cell Sci 108: 25-35.
34. Yanisch-Perron C, Vieira J, Messing J. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119.
35. Zheng L, Szanislo PJ. 1999. Cloning and use of the WdURA5 gene as hisG cassette selection marker for potentially disrupting multiple genes in Wangiella dermatitidis. Med Mycol 37: 85-96.