Global characterization of in vivo enzyme catalytic rates and their correspondence to in vitro kcat measurements

Proceedings of the National Academy of Sciences of the United States of America

Volumn 113, Issue 12, 2016, Pages 3401-3406

  Davidia, Dan ^a   Noorb, Elad ^b   Liebermeisterc, Wolfram ^c   Bar Evend, Arren ^d   Flamholze, Avi ^e   Tummlerf, Katja ^f   Barenholza, Uri ^a   Goldenfelda, Miki ^a   Shlomig, Tomer ^g   Miloa, Ron ^a  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^b ETH ZURICH   (Switzerland)

^c CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^d MAX PLANCK INSTITUTE OF MOLECULAR PLANT PHYSIOLOGY   (Germany)

^e UNIVERSITY OF CALIFORNIA   (United States)

^f HUMBOLDT UNIVERSITY   (Germany)

^g TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

Author keywords

Flux balance analysis; kcat; Kinetic constants; Proteomics; Turnover number

Indexed keywords

AMINO ACID METABOLISM; ANALYSIS; ARTICLE; BACTERIAL GENOME; CARBON SOURCE; CATALYSIS; CITRIC ACID CYCLE; CORRELATION ANALYSIS; ENZYME ACTIVE SITE; ESCHERICHIA COLI; GLYCOLYSIS; HIGH THROUGHPUT SCREENING; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; NUCLEOTIDE METABOLISM; PRIORITY JOURNAL; PROTEOMICS; STOICHIOMETRY; THERMODYNAMICS; TURNOVER NUMBER; METABOLISM;

ENZYME;

CATALYSIS; ENZYMES;

EID: 84962212496     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1514240113     Document Type: Article

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