Determining antioxidant activities of lactobacilli cell-free supernatants by cellular antioxidant assay: A comparison with traditional methods

PLoS ONE

Volumn 10, Issue 3, 2015, Pages

  Xing, Jiali ^a   Wang, Gang ^a   Zhang, Qiuxiang ^a   Liu, Xiaoming ^a   Gu, Zhennan ^a   Zhang, Hao ^a   Chen, Yong Q ^a   Chen, Wei ^a,b  

^a JIANGNAN UNIVERSITY   (China)

^b Synergetic Innovation Center of Food Safety and Nutrition   (China)

Author keywords

[No Author keywords available]

Indexed keywords

PROBIOTIC AGENT; 1,1-DIPHENYL-2-PICRYLHYDRAZYL; ANTIOXIDANT; BIPHENYL DERIVATIVE; HYDROXYL RADICAL; LINOLEIC ACID; PICRIC ACID; SCAVENGER;

ANTIOXIDANT ACTIVITY; ANTIOXIDANT ASSAY; ARTICLE; BACTERIAL STRAIN; CELL FREE SYSTEM; CELLULAR ANTIOXIDANT ACTIVITY ASSAY; CONTROLLED STUDY; CYTOTOXICITY; DPPH RADICAL SCAVENGING ASSAY; HEPG2 CELL LINE; HUMAN; HUMAN CELL; HYDROXYL RADICAL SCAVENGING ASSAY; INHIBITION OF LINOLEIC ACID PEROXIDATION ASSAY; INTERMETHOD COMPARISON; LACTOBACILLUS; LACTOBACILLUS ACIDOPHILUS; LACTOBACILLUS CASEI; LACTOBACILLUS FERMENTUM; LACTOBACILLUS PLANTARUM; LACTOBACILLUS REUTERI; LACTOBACILLUS RHAMNOSUS; NONHUMAN; REDUCING POWER ASSAY; SUPERNATANT; BIOASSAY; CHEMISTRY; COMPARATIVE STUDY; HEP-G2 CELL LINE; LIPID PEROXIDATION; METABOLISM; OXIDATION REDUCTION REACTION; PROCEDURES;

LACTOBACILLUS; LACTOBACILLUS RHAMNOSUS;

ANTIOXIDANTS; BIOLOGICAL ASSAY; BIPHENYL COMPOUNDS; CELL-FREE SYSTEM; FREE RADICAL SCAVENGERS; HEP G2 CELLS; HUMANS; HYDROXYL RADICAL; LACTOBACILLUS; LINOLEIC ACID; LIPID PEROXIDATION; OXIDATION-REDUCTION; PICRATES;

EID: 84961291437     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0119058     Document Type: Article

References (56)

1. Cederbaum AI, Lu Y, Wu D. Role of oxidative stress in alcohol-induced liver injury. Arch Toxicol. 2009; 83: 519-548. doi: 10.1007/s00204-009-0432-0 PMID: 19448996
2. Nobili V, Parola M, Alisi A, Marra F, Piemonte F, Mombello C, et al. Oxidative stress parameters in paediatric non-alcoholic fatty liver disease. Int J Mol Med. 2010; 26: 471-476. PMID: 20818484
3. Beckman KB, Ames BN. The free radical theory of aging matures. Physiol Rev. 1998; 78: 547-581. PMID: 9562038
4. Yu JH, Kim H. Oxidative stress and cytokines in the pathogenesis of pancreatic cancer. J Cancer Pre. 2014; 19: 97-102. doi: 10.15430/JCP.2014.19.2.97 PMID: 25337577
5. Halliwell B, Gutteridge J. Free radicals in biology and medicine. Oxford Clarendon Press; 1985. pp. 23-30.
6. Sies H. Strategies of Antioxidant Defense. Eur J Biochem. 1993; 215: 213-219. PMID: 7688300
7. Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem. 1998; 46: 4113-4117.
8. Liu C, Pan T. In Vitro Effects of Lactic Acid Bacteria on Cancer Cell Viability and Antioxidant Activity. J Food Drug Anal. 2010; 18: 77-86.
9. Choi SS, Kim Y, Han KS, You S, Oh S, Kim SH. Effects of Lactobacillus strains on cancer cell proliferation and oxidative stress in vitro. Lett Appl Microbiol. 2006; 42: 452-458. PMID: 16620202
10. Lee J, Hwang KT, Heo MS, Lee JH, Park KY. Resistance of Lactobacillus plantarum KCTC 3099 from Kimchi to oxidative stress. J Med Food. 2005; 8: 299-304. PMID: 16176138
11. Forsyth CB, Farhadia A, Jakate SM, Tang YM, Shaikh M, Keshavarzian A. Lactobacillus GG treatment ameliorates alcohol-induced intestinal oxidative stress, gut leakiness, and liver injury in a rat model of alcoholic steatohepatitis. Alcohol. 2009; 43: 163-172. doi: 10.1016/j.alcohol.2008.12.009 PMID: 19251117
12. Chen P, Zhang Q, Dang H, Liu X, Tian F, Zhao J, et al. Screening for potential new probiotic based on probiotic properties and α-glucosidase inhibitory activity. Food Control. 2014; 35: 65-72.
13. Zhang S, Liu L, Su Y, Li H, Sun Q, Liang X, et al. Antioxidative activity of lactic acid bacteria in yogurt. Afr J Microbiol Res. 2011; 5: 5194-5201.
14. Lin M-Y, Yen C-L. Antioxidative Ability of Lactic Acid Bacteria. J Agric Food Chem. 1999; 47: 1460-1466. PMID: 10563999
15. Zhang L, Liu C, Li D, Zhao Y, Zhang X, Zeng X, et al. Antioxidant activity of an exopolysaccharide isolated from Lactobacillus plantarum C88. Int J Biol Macromol. 2013; 54: 270-275. doi: 10.1016/j.ijbiomac.2012.12.037 PMID: 23274679
16. Zhang J, Wang D, Zhang J, Wang Y-H. Extraction and antioxidant activity of the soluble protein from Lactobacillus rhamnosus broth. Food Sci Technol. 2012; 37: 235-239.
17. Bing SR, Kinouchi T, Kataoka K, Kuwahara T, Ohnishi Y. Protective effects of a culture supernatant of Lactobacillus acidophilus and antioxidants on ileal ulcer formation in rats treated with a nonsteroidal antiinflammatory drug. Microbiol Immunol. 1998; 42: 745-753. PMID: 9886147
18. Wang YH, Liu YL, Sidhu A, Ma ZH, McClain C, Feng WK. Lactobacillus rhamnosus GG culture supernatant ameliorates acute alcohol-induced intestinal permeability and liver injury. Am J Physiol-Gastroint Liver Physiol. 2012; 303: G32-G41. doi: 10.1152/ajpgi.00024.2012 PMID: 22538402
19. Shen Q, Shang N, Li P. In vitro and in vivo antioxidant activity of Bifidobacterium animalis 01 isolated from centenarians. Curr Microbiol. 2011; 62: 1097-1103. doi: 10.1007/s00284-010-9827-7 PMID: 21132298
20. Wang X, Luo X, Xu X, Yu M, Yu C, Jiang N, et al. Comparative Studies on Antioxidant Activities of Different Lactic Acid Bacterial Strains. Food science. 2010; 31: 197-201.
21. Kanno T, Kuda T, An C, Takahashi H, Kimura B. Radical scavenging capacities of saba-narezushi, Japanese fermented chub mackerel, and its lactic acid bacteria. LWT-Food Sci Technol. 2012; 47: 25-30.
22. Lin MY, Yen CL. Reactive Oxygen Species and Lipid Peroxidation Product-Scavenging Ability of Yogurt Organisms. J Dairy Sci. 1999; 82: 1629-1634. PMID: 10480088
23. Apak R, Gueclue K, Demirata B, Oezyuerek M, Celik SE, Bektasoglu B, et al. Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules. 2007; 12: 1496-1547. PMID: 17909504
24. Niki E. Assessment of antioxidant capacity in vitro and in vivo. Free Radic Biol Med. 2010; 49: 503-515. doi: 10.1016/j.freeradbiomed.2010.04.016 PMID: 20416370
25. López-Alarcón C, Denicola A. Evaluating the antioxidant capacity of natural products: A review on chemical and cellular-based assays. Anal Chim Acta. 2013; 763: 1-10. doi: 10.1016/j.aca.2012.11.051 PMID: 23340280
26. Wolfe KL, Liu RH. Cellular antioxidant activity (CAA) assay for assessing antioxidants, foods, and dietary supplements. J Agric Food Chem. 2007; 55: 8896-8907. PMID: 17902627
27. Sun J, Hu XL, Le GW, Shi YH. Lactobacilli prevent hydroxy radical production and inhibit Escherichia coli and Enterococcus growth in system mimicking colon fermentation. 2010; 50: 264-269. doi: 10.1111/j.1472-765X.2009.02786.x PMID: 20059670
28. Lin M, Chang F. Antioxidative effect of intestinal bacteria Bifidobacterium longum ATCC 15708 and Lactobacillus acidophilus ATCC 4356. Dig Dis Sci. 2000; 45: 1617-1622. PMID: 11007114
29. Persichetti E, De Michele A, Codini M, Traina G. Antioxidative capacity of Lactobacillus fermentum LF31 evaluated in vitro by oxygen radical absorbance capacity assay. Nutrition. 2014; 30: 936-938. doi: 10.1016/j.nut.2013.12.009 PMID: 24985014
30. Lee J, Hwang KT, Chung MY, Cho DH, Park CS. Resistance of Lactobacillus casei KCTC 3260 to reactive oxygen species (ROS): Role for a metal ion chelating effect. J Food Sci. 2005; 70: M388-M391.
31. Wang J, Ji HF, Wang SX, Zhang DY, Liu H, Shan DC, et al. Lactobacillus plantarum ZLP001: In vitro Assessment of Antioxidant Capacity and Effect on Growth Performance and Antioxidant Status in Weaning Piglets. Asian Australas J Anim Sci. 2012; 25: 1153-1158. doi: 10.5713/ajas.2012.12079 PMID: 25049675
32. Hathout AS, Mohamed SR, El-Nekeety AA, Hassan NS, Aly SE, Abdel-Wahhab MA. Ability of Lactobacillus casei and Lactobacillus reuteri to protect against oxidative stress in rats fed aflatoxins-contaminated diet. Toxicon. 2011; 58: 179-186. doi: 10.1016/j.toxicon.2011.05.015 PMID: 21658402
33. Bermudez-Brito M, Munoz-Quezada S, Gomez-Llorente C, Matencio E, Bernal MJ, Romero F, et al. Cell-Free Culture Supernatant of Bifidobacterium breve CNCM I-4035 Decreases Pro-Inflammatory Cytokines in Human Dendritic Cells Challenged with Salmonella typhi through TLR Activation. PLoS One. 2013; 8:1-8.
34. Gazi MR, Yokota M, Tanaka Y, Kanda S, Itabashi H. Effects of protozoa on the antioxidant activity in the ruminal fluid and blood plasma of cattle. Anal Chim Acta. 2007; 78: 34-40.
35. Lin MY, Yen CL. Antioxidative ability of lactic acid bacteria. 1999; 47: 1460-1466. PMID: 10563999
36. Wang L, Chen J, Xie H, Ju X, Liu RH. Phytochemical profiles and antioxidant activity of adlay varieties. J Agric Food Chem. 2013; 61: 5103-5113. doi: 10.1021/jf400556s PMID: 23647066
37. Felice DL, Sun J, Liu RH. A modified methylene blue assay for accurate cell counting. J Funct Food. 2009; 1: 109-118.
38. Malta LG, Tessaro EP, Eberlin M, Pastore GM, Liu RH. Assessment of antioxidant and antiproliferative activities and the identification of phenolic compounds of exotic Brazilian fruits. Food Res Int. 2013; 53: 417-425.
39. Faller ALK, Fialho E, Liu RH. Cellular Antioxidant Activity of Feijoada Whole Meal Coupled with an in Vitro Digestion. J Agric Food Chem. 2012; 60: 4826-4832. doi: 10.1021/jf300602w PMID: 22509740
40. Huang DJ, Ou BX, Prior RL. The chemistry behind antioxidant capacity assays. J Agric Food Chem. 2005; 53: 1841-1856. PMID: 15769103
41. Meir S, Kanner J, Akiri B, Philosoph-Hadas S. Determination and Involvement of Aqueous Reducing Compounds in Oxidative Defense Systems of Various Senescing Leaves. J Agric Food Chem. 1995; 43: 1813-1819.
42. Lin M-Y, Yen C-L. Inhibition of Lipid Peroxidation by Lactobacillus acidophilus and Bifidobacterium longum. J Agric Food Chem. 1999; 47: 3661-3664. PMID: 10552700
43. Zhu YP, Fan JF, Cheng YQ, Li LT. Improvement of the antioxidant activity of Chinese traditional fermented okara (Meitauza) using Bacillus subtilis B2. Food Control. 2008; 19: 654-661.
44. Tabart J, Franck T, Kevers C, Pincemail J, Serteyn D, Defraigne JO, et al. Antioxidant and anti-inflammatory activities of Ribes nigrum extracts. Food Chemistry. 2012; 131: 1116-1122.
45. Yi Y, Huang F, Zhang MW, Zhang RF, Deng YY, Wei ZC, et al. Solution Properties and in Vitro Anti-Tumor Activities of Polysaccharides from Longan Pulp. Molecules. 2013; 18: 11601-11613. doi: 10.3390/molecules180911601 PMID: 24051475
46. Yang J, Liu RH. Synergistic Effect of Apple Extracts and Quercetin 3-beta-D-Glucoside Combination on Antiproliferative Activity in MCF-7 Human Breast Cancer Cells in Vitro. J Agric Food Chem. 2009; 57: 8581-8586. doi: 10.1021/jf8039796 PMID: 19694432
47. Song W, Derito CM, Liu MK, He XJ, Dong M, Liu RH. Cellular Antioxidant Activity of Common Vegetables. J Agric Food Chem. 2010; 58: 6621-6629. doi: 10.1021/jf9035832 PMID: 20462192
48. Maudsdotter L, Jonsson H, Roos S, Jonsson AB. Lactobacilli reduce cell cytotoxicity caused by Streptococcus pyogenes by producing lactic acid that degrades the toxic component lipoteichoic acid. Antimicrob Agents Chemother. 2011; 55: 1622-1628. doi: 10.1128/AAC.00770-10 PMID: 21245448
49. Li WI, Brackett BG, Halper J. Culture supernatant of Lactobacillus acidophilus stimulates proliferation of embryonic cells. Exp Biol Med. 2005; 230: 494-500. PMID: 15985625
50. Hirawan R, Diehl-Jones W, Beta T. Comparative Evaluation of the Antioxidant Potential of Infant Cereals Produced from Purple Wheat and Red Rice Grains and LC-MS Analysis of Their Anthocyanins. J Agric Food Chem. 2011; 59: 12330-12341. doi: 10.1021/jf202662a PMID: 22035073
51. Li WX, Li YF, Zhai YJ, Chen WM, Kurihara H, He RR. Theacrine, a Purine Alkaloid Obtained from Camellia assamica var. kucha, Attenuates Restraint Stress-Provoked Liver Damage in Mice. J Agric Food Chem. 2013; 61: 6328-6335. doi: 10.1021/jf400982c PMID: 23678853
52. Asikin Y, Takahashi M, Mishima T, Mizu M, Takara K, Wada K. Antioxidant activity of sugarcane molasses against 2,2′-azobis(2-amidinopropane) dihydrochloride-induced peroxyl radicals. Food Chemistry. 2013; 141: 466-472. doi: 10.1016/j.foodchem.2013.03.045 PMID: 23768381
53. Mustapha N, Bouhlel I, Chaabane F, Bzeouich IM, Ghedira K, Hennebelle T, et al. Aqueous extract of Crataegus azarolus protects against DNA damage in human lymphoblast Cell K562 and enhances antioxidant activity. Appl Biochem Biotechnol. 2014; 172: 2266-2275. doi: 10.1007/s12010-013-0667-3 PMID: 24347159
54. Puertollano MA, Puertollano E, Alvarez de Cienfuegos G, de Pablo MA. Culture Supernatants from Lactobacillus plantarum Induce Necrosis on a Human Promyelocytic Leukemia Cell Line. P Nutr Soc. 2013; 13: 195-203(9).
55. Ramesh V, Kumar R, Singh RRB, Kaushik JK, Mann B. Comparative evaluation of selected strains of lactobacilli for the development of antioxidant activity in milk. Dairy Sci Technol. 2012; 92: 179-188.
56. Huang H, Sun Y, Lou S, Li H, Ye X. In vitro digestion combined with cellular assay to determine the antioxidant activity in Chinese bayberry (Myrica rubra Sieb. et Zucc.) fruits: a comparison with traditional methods. Food chemistry. 2014; 146: 363-370. doi: 10.1016/j.foodchem.2013.09.071 PMID: 24176355