Inactivation of GDP-fucose transporter gene (Slc35c1) in CHO cells by ZFNs, TALENs and CRISPR-Cas9 for production of fucose-free antibodies

Biotechnology Journal

Volumn 11, Issue 3, 2016, Pages 399-414

  Chan, Kah Fai ^a,b   Shahreel, Wahyu ^a   Wan, Corrine ^a   Teo, Gavin ^a   Hayati, Noor ^a   Tay, Shi Jie ^a   Tong, Wen Han ^a   Yang, Yuansheng ^a   Rudd, Pauline M ^a   Zhang, Peiqing ^a,b   Song, Zhiwei ^a,b  

^a AGENCY FOR SCIENCE   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Chinese hamster ovary (CHO) cells; Fluorescence activated cell sorting (FACS); Fucose free antibodies; GDP fucose transporter gene (Slc35c1); Genome editing technologies

Indexed keywords

CELLS; CYTOLOGY; CYTOTOXICITY; FLOW CYTOMETRY; GENES; MASS SPECTROMETRY;

ANTIBODY-DEPENDENT CELLULAR CYTOTOXICITIES; CHINESE HAMSTER OVARY CELLS; EDITING TECHNOLOGY; FLUORESCENCE ACTIVATED CELL SORTING; FUCOSYLTRANSFERASES; MASS SPECTROMETRY ANALYSIS; N-GLYCANS; TRANSPORTER GENES;

ANTIBODIES;

ANTIBODY; CARRIER PROTEIN; FUCOSYLTRANSFERASE; GDP FUCOSE TRANSPORTER; GENOMIC DNA; GLYCAN; LECTIN; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR NUCLEASE; UNCLASSIFIED DRUG; ZINC FINGER NUCLEASE; ERYTHROPOIETIN; FC RECEPTOR; FUCOSE; GLUCOSE TRANSPORTER; HYBRID PROTEIN; MONOCLONAL ANTIBODY; ZINC FINGER PROTEIN;

ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; CELL GROWTH; CELL ISOLATION; CELL MUTANT; CHO CELL LINE; CODON; CRISPR CAS SYSTEM; DNA BINDING; FEMALE; FLUORESCENCE ACTIVATED CELL SORTING; GENE INACTIVATION; GENETIC COMPLEMENTATION; HUMAN; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; TRANSIENT EXPRESSION; ANIMAL; BIOSYNTHESIS; CHEMISTRY; CRICETULUS; FLOW CYTOMETRY; GENE SILENCING; GENETICS; HAMSTER; METABOLISM; MUTATION;

ANIMALS; ANTIBODIES, MONOCLONAL; CHO CELLS; CRICETINAE; CRICETULUS; CRISPR-CAS SYSTEMS; ERYTHROPOIETIN; FLOW CYTOMETRY; FUCOSE; GENE SILENCING; HUMANS; MONOSACCHARIDE TRANSPORT PROTEINS; MUTATION; RECEPTORS, IGG; RECOMBINANT FUSION PROTEINS; ZINC FINGERS;

EID: 84959236890     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201500331     Document Type: Article

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