RCA-I-resistant CHO mutant cells have dysfunctional GnT I and expression of normal GnT I in these mutants enhances sialylation of recombinant erythropoietin

Metabolic Engineering

Volumn 12, Issue 4, 2010, Pages 360-368

  Goh, John S Y ^a   Zhang, Peiqing ^a   Fai Chan, Kah ^a   May Lee, May ^a   Fee Lim, Sing ^a   Song, Zhiwei ^a  

^a AGENCY FOR SCIENCE   (Singapore)

Author keywords

CHO glycosylation mutants; Isoelectric focusing (IEF); N acetylglucosaminyltransferase I (GnT I); Recombinant erythropoietin (EPO); Ricinus communis agglutinin I (RCA I); Sialylation

Indexed keywords

AMINO ACID SUBSTITUTION; BASE PAIRS; BIOTECHNOLOGY INDUSTRY; CHO CELL; COMPLEMENTATION; GLYCAN STRUCTURES; ISOELECTRIC FOCUSING; MUTANT CELLS; MUTANT LINES; N-GLYCANS; NEW STRATEGY; RECOMBINANT ERYTHROPOIETIN (EPO); RICINUS COMMUNIS; SEQUENCING ANALYSIS; SIALYLATION; WILD TYPES;

AMINO ACIDS; ESTERIFICATION; GLYCOSYLATION; ORGANIC ACIDS;

ELECTROPHORESIS;

AGGLUTININ; COMPLEMENTARY DNA; GLYCAN; N ACETYLGLUCOSAMINYLTRANSFERASE; RECOMBINANT ERYTHROPOIETIN;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; BASE PAIRING; BIOTECHNOLOGY; CARBOHYDRATE ANALYSIS; CELL MUTANT; CHO CELL; CONTROLLED STUDY; DNA ISOLATION; DRUG SYNTHESIS; FEMALE; GENE MUTATION; GENETIC COMPLEMENTATION; GENETIC TRANSFECTION; GLYCOSYLATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; RICINUS COMMUNIS; SEQUENCE ANALYSIS; SIALYLATION; STOP CODON; WILD TYPE;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; ERYTHROPOIETIN, RECOMBINANT; GLYCOSYLATION; N-ACETYLGLUCOSAMINYLTRANSFERASES; N-ACETYLNEURAMINIC ACID; PLANT LECTINS; POINT MUTATION;

RICINUS COMMUNIS;

EID: 77953025411     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2010.03.002     Document Type: Article

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