Adaptable stirred-tank culture strategies for large scale production of multicellular spheroid-based tumor cell models

Journal of Biotechnology

Volumn 221, Issue , 2016, Pages 118-129

  Santo, Vítor E ^a,b   Estrada, Marta F ^a,b   Rebelo, Sofia P ^a,b   Abreu, Sofia ^a,b   Silva, Inês ^a,b   Pinto, Catarina ^a,b   Veloso, Susana C ^a,b   Serra, Ana Teresa ^a,b   Boghaert, Erwin ^c   Alves, Paula M ^a,b   Brito, Catarina ^a,b  

^a INSTITUTO DE BIOLOGIA EXPERIMENTAL E TECNOLÓGICA   (Portugal)

^b INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^c ABBVIE INC   (United States)

Author keywords

3D cell culture; Drug discovery; In vitro cancer models; Stirred tank culture systems; Tumor spheroids

Indexed keywords

CELL CULTURE; CELLS; CYTOLOGY; DISEASES; DRUG PRODUCTS; IMAGING TECHNIQUES; TANKS (CONTAINERS); TUMORS;

3-D CELL CULTURE; CANCER MODELS; DRUG DISCOVERY; STIRRED TANK; TUMOR SPHEROID;

DIAGNOSIS;

ALGINIC ACID;

ARTICLE; CANCER CELL; CANCER CELL LINE; CANCER MODEL; CELL PROLIFERATION; CELL STRUCTURE; CELL VIABILITY; CLINICAL PROTOCOL; CONTINUOUS STIRRED TANK REACTOR; DRUG SCREENING; FIBROBLAST; HUMAN; HUMAN CELL; HYDRODYNAMICS; HYPOXIA; LARGE SCALE PRODUCTION; MICROENCAPSULATION; MULTICELLULAR SPHEROID; PRIORITY JOURNAL; RELIABILITY; REPRODUCIBILITY; TUMOR CELL CULTURE; TUMOR CELL LINE; BATCH CELL CULTURE; BIOLOGICAL MODEL; CELL SURVIVAL; COCULTURE; CYTOLOGY; PROCEDURES;

BATCH CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; CELL PROLIFERATION; CELL SURVIVAL; COCULTURE TECHNIQUES; FIBROBLASTS; HUMANS; HYDRODYNAMICS; MODELS, BIOLOGICAL; REPRODUCIBILITY OF RESULTS; SPHEROIDS, CELLULAR;

EID: 84957944330     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2016.01.031     Document Type: Article

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