Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types

Biotechnology and Bioengineering

Volumn 83, Issue 2, 2003, Pages 173-180

  Kelm, Jens M ^a,b   Timmins, Nicholas E ^a   Brown, Catherine J ^a   Fussenegger, Martin ^b   Nielsen, Lars K ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b ETH ZURICH   (Switzerland)

Author keywords

Multicellular spheroid; Multicellular tumor spheroids (MCTS); Organotypic; Solid tumor; Three dimensional (3D) cell culture

Indexed keywords

CELLS; METABOLISM; PHYSIOLOGY; STRUCTURAL ANALYSIS;

MULTICELLULAR TUMOR SPHEROIDS (MCTS);

TUMORS;

ARTICLE; ARTIFICIAL ORGAN; CELL CULTURE; CELL STRAIN HEPG2; CELL STRAIN MCF 7; CELL STRUCTURE; CELL TYPE; HUMAN; HUMAN CELL; METABOLISM; MODEL; MULTICELLULAR SPHEROID; MULTICELLULAR TUMOR SPHEROID; ORGANOTYPIC MODEL; PHYSIOLOGY; SOLID TUMOR; STRUCTURE ANALYSIS; TECHNIQUE; THREE DIMENSIONAL CELL CULTURE; TOXICOLOGY; TUMOR;

BREAST NEOPLASMS; CARCINOMA, HEPATOCELLULAR; CELL CULTURE TECHNIQUES; CELL DEATH; FEMALE; HUMANS; LIVER NEOPLASMS; MICROSCOPY, ELECTRON; MICROSCOPY, ELECTRON, SCANNING; MODELS, BIOLOGICAL; NEOPLASMS; TUMOR CELLS, CULTURED;

MINK CELL FOCUS-FORMING VIRUS;

EID: 0037701271     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10655     Document Type: Article

References (37)

1. Buffa FM, West C, Byrne K, Moore JV, Nahum AE. 2001. Radiation response and cure rate of human colon adenocarcinoma spheroids of different size: The significance of hypoxia on tumour control modelling. Int J Radiat Oncol Biol Phys 49:1109-1118.
2. Castañeda F, Kinne RKH. 2000. Short exposure to millimolar concentrations of ethanol iduces apoptic cell death in multicellular HepG2 spheroids. J Cancer Res Clin Oncol 126:305-310.
3. Chignola R, Schenetti A, Andrighetto G, Chiesa E, Foroni R, Sartoris S, Tridente G, Liberati D. 2000. Forecasting the growth of multicell tumour spheroids: Implications for the dynamic growth of solid tumours. Cell Prolif 33:219-229.
4. Dertinger H, Hulser DF. 1984. Intercellular communication in spheroids. Recent Results Cancer Res 95:67-83.
5. Desoize B. 2000. Contribution of three-dimensional culture to cancer research. Crit Rev Oncol Hematol 36:59-60.
6. Desoize B, Jardillier J. 2000. Multicellular resistance: A paradigm for clinical resistance? Crit Rev Oncol Hematol 36:193-207.
7. Dubessy C, Merlin JM, Marchal C, Guillemin F. 2000. Spheroids in radiobiology and photodynamic therapy. Crit Rev Oncol Hematol 36:179-192.
8. Düchting W, Vogelsaenger T. 1984. Modelling and simulation of growing spheroids. Recent Results Cancer Res 95:168-179.
9. Fracasso G, Colombatti M. 2000. Effect of therapeutic macromolecules in spheroids. Crit Rev Oncol Hematol 36:159-178.
10. Hamilton G. 1998. Multicellular spheroids as an in vitro tumor model. Cancer Lett 131:29-34.
11. Hauptmann S, Denkert C, Lohrke H, Tietze L, Ott S, Klosterhalfen B, Mittermayer C. 1995. Integrin expression on colorectal tumor-cells growing as monolayers, as multicellular tumor spheroids, or in nudemice. Int J Cancer 61:819-825.
12. Inch WR, McCredie JA, Sutherland RM. 1970. Growth of nodular carcinomas in rodents compared with multi-cell spheroids in tissue culture. Growth 34:271-282.
13. Karbach U, Gerharz CD, Groebe K, Gabbert HE, Muellerklieser W. 1992. Rhabdomyosarcoma spheroids with central proliferation and differentiation. Cancer Res 52:474-477.
14. Kawata M, Sekiya S, Kera K, Kimura H, Takamizawa H. 1991. Neural rosette formation within in vitro spheroids of a clonal human terato-carcinoma cell-line, pa-1/nr - role of extracellular-matrix components in the morphogenesis. Cancer Res 51:2655-2669.
15. Keller GM. 1995. In vitro differentiation of embryonic stem cells. Curr Opin Cell Biol 7:862-869.
16. Khalil M, Shariat-Panahi A, Tootle R, Ryder T, McCloskey P, Roberts E, Hodgson H, Selden C. 2001. Human hepatocyte cell lines proliferating as cohesive spheroid colonies in alginate markedly upregulate both synthestic and detoxifactory liver function. J Hepatol 297:68-77.
17. Knuechel R, Keng P, Hofstaedter F, Langmuir V, Sutherland RM, Penney DP. 1990. Differentiation patterns in 2-dimensional and 3-dimensional culture systems of human squamous carcinoma cell-lines. Am J Pathol 137:725-736.
18. Knuechel R, Siebert-Wellnhofer A, Traub O, Dermietzel R. 1996. Connexin expression and intercellular communication in two- and three-dimensional in vitro cultures of human bladder carcinoma. Am J Pathol 149:1321-1332.
19. Kunz-Schughart LA, Kreutz M, Knuechel R. 1998. Multicellular spheroids: A three-dimensional in vitro culture system to study tumour biology. Int J Exp Pathol 79:1-23.
20. Kunz-Schughart LA, Mueller-Klieser W. 2000. Three-dimensional culture. In: Masters JRW, editor. Animal cell culture: A practical approach, 3rd edition. New York: Oxford University Press. p 123-184.
21. Landry J, Freyer JP. 1984. Regulatory mechanisms in spheroidal aggregates of normal and cancerous cells. Recent Results Cancer Res 95:50-66.
22. Li G, Satyamoortthy K, Herlyn M. 2001. N-cadherin-mediated intercellular interactions promote survival and migration of melanoma cells. Cancer Res 61:3819-3825.
23. Marusic M, Bajzer Z, Vuk-Pavlovic, Freyer JP. 1994. Tumour growth in vivo and as multicellular spheroids compared by mathematical models. Bull Math Biol 56:617-631.
24. May MH, Sefton MV. 1999. Conformal coating of small particles and cell aggregates at a liquid-liquid interface. Bioartif Organs II Technol Med Mater 875:126-134.
25. Mueller-Klieser W. 1997. Three-dimensional cell cultures: From molecular mechanisms to clinical applications. Am J Physiol 273:C1109-C1023.
26. Mueller-Klieser W. 2000. Tumor biology and experimental therapeutics. Crit Rev Oncol Hematol 36:123-139.
27. Nederman T, Twentyman P. 1984. Spheroids for studies of drug effects. Recent Results Cancer Res 95:84-102.
28. Offner FA, Ott G, Povey S, Knuechel R, Preisler V, Fuezesi L, Klosterhalfen B, Ruebben H, Hofstaedter F, Kirkpatrick CJ. 1991. Characterization of the new bladder-cancer cell-line Hok-1 - Expression of transitional, squamous and glandular differentiation patterns. Int J Cancer 49:122-128.
29. Paulus W, Huettner C, Tonn JC. 1994. Collagens, integrins and the mesenchymal drift in glioblastomas - A comparison of biopsy specimens, spheroid and early monolayer-cultures. Int J Cancer 58:841-846.
30. Peshwa MV, Wu FJ, Sharp HL, Cerra FB, Hu WS. 1996. Mechanistics of formation and ultrastructural evaluation of hepatocyte spheroids. In Vitro Cell Devel Biol Anim 32:197-203.
31. Pettet GJ, Please CP, Tindall MJ, McElwain DLS. 2001. The migration of cells in multicell tumor spheroids. Bull Math Biol 63:231-257.
32. Santini MT, Rainaldi G, Indovina PL. 1999. Multicellular tumour spheroids in radiation biology. Int J Radiat Biol 75:787-799.
33. Sutherland RM. 1988. Cell and environment interactions in tumor microregions: The multicell spheroids model. Science 240:177-184.
34. Thompson J, Parsons P, Sturm R. 1993. In vivo and in vitro expression of octamer binding proteins in human melanoma metastases, brain tissue and fibroblasts. Pigment Cell Res 6:13-22.
35. Waleh NS, Gallo J, Grant TD, Murphy BJ, Kramer RH, Sutherland RM. 1994. Selective down-regulation of integrin receptors in spheroids of squamous-cell carcinoma. Cancer Res 54:838-843.
36. Walenta S, Doetsch J, Mueller-Klieser W, Kunz-Schughart LA. 2000. Metabolic imaging in multicellular spheroids of oncogene-transfected fibroblasts. J Histochem Cytochem 48:509-522.
37. Yamada K, Kamihira M, Hamamoto R, Iijima S. 1998. Efficient induction of hepatocyte spheroids in a suspension culture using a water-soluble synthetic polymer as an artificial matrix. J Biochem (Tokyo) 123:1017-1023.