Recombinase-mediated cassette exchange (RMCE) for monoclonal antibody expression in the commercially relevant CHOK1SV cell line

Biotechnology Progress

Volumn 31, Issue 6, 2015, Pages 1645-1656

  Zhang, Lin ^a   Inniss, Mara C ^a   Han, Shu ^a   Moffat, Mark ^a   Jones, Heather ^a   Zhang, Baohong ^a   Cox, Wendy L ^b   Rance, James R ^b   Young, Robert J ^c  

^a PFIZER INC   (United States)

^b LONZA AG   (Switzerland)

^c Lonza Biologics   (United Kingdom)

Author keywords

Cell line development; Monoclonal antibody; RMCE; Site specific integration; Stability

Indexed keywords

ANTIBODIES; CELL CULTURE; CELLS; CLONING; CONVERGENCE OF NUMERICAL METHODS; CYTOLOGY; GENE EXPRESSION; GENES; MONOCLONAL ANTIBODIES; PRODUCTIVITY; STABILITY;

AMERICAN INSTITUTE OF CHEMICAL ENGINEERS; CELL LINES; EXPRESSION CASSETTE; GENERATION NUMBERS; LONG TERM PRODUCTION; RMCE; SITE-SPECIFIC; THERAPEUTIC MONOCLONAL ANTIBODIES;

CLONE CELLS;

DNA POLYMERASE; MONOCLONAL ANTIBODY; RECOMBINASE;

ANIMAL; CELL LINE; CHO CELL LINE; CRICETULUS; GENE VECTOR; GENETICS; HAMSTER; METABOLISM; MOLECULAR CLONING; PROCEDURES;

ANIMALS; ANTIBODIES, MONOCLONAL; CELL LINE; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; CRICETULUS; DNA NUCLEOTIDYLTRANSFERASES; GENETIC VECTORS;

EID: 84956842466     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.2175     Document Type: Article

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