Generation of stable cell lines by site-specific integration of transgenes into engineered Chinese hamster ovary strains using an FLP-FRT system

Journal of Biotechnology

Volumn 147, Issue 2, 2010, Pages 122-129

  Zhou, Hong ^a   Liu, Zhi gang ^a   Sun, Zhi wei ^a   Huang, Ying ^a   Yu, Wei yuan ^a  

^a BEIJING INSTITUTE OF BIOTECHNOLOGY   (China)

Author keywords

Chinese hamster ovary (CHO); Recombinant protein; Site specific integration; Transcriptionally active sites

Indexed keywords

CHINESE HAMSTER OVARY; RECOMBINANT PROTEIN; SITE-SPECIFIC; SITE-SPECIFIC INTEGRATION; TRANSCRIPTIONALLY ACTIVE;

AMPLIFICATION; CELL CULTURE; CLONE CELLS; CLONING; PHOSPHATASES; PROTEINS;

GENE EXPRESSION;

ALKALINE PHOSPHATASE; DIHYDROFOLATE REDUCTASE; ERYTHROPOIETIN; GENOMIC DNA; KANAMYCIN KINASE; METHOTREXATE; RECOMBINANT PROTEIN; RECOMBINASE; TISSUE PLASMINOGEN ACTIVATOR;

ANIMAL CELL; ARTICLE; CELL LINE; CELL SELECTION; CELL STRAIN; CHO CELL; CONTROLLED STUDY; GENE AMPLIFICATION; GENE EXPRESSION; GENE SEQUENCE; GENE TARGETING; GENETIC ENGINEERING; MOLECULAR CLONING; NONHUMAN; PLASMID; PRIORITY JOURNAL; RECOMBINANT DNA TECHNOLOGY; RECOMBINANT GENE; REPORTER GENE; STABLE EXPRESSION; TRANSGENE;

ALKALINE PHOSPHATASE; ANIMALS; BLOTTING, SOUTHERN; CHO CELLS; CINNAMATES; CRICETINAE; CRICETULUS; DNA NUCLEOTIDYLTRANSFERASES; ERYTHROPOIETIN; GENE AMPLIFICATION; GENE TARGETING; GENE TRANSFER TECHNIQUES; GENETIC ENGINEERING; GENETIC VECTORS; HUMANS; HYGROMYCIN B; ISOENZYMES; METHOTREXATE; PLASMIDS; PROTEIN STABILITY; RECOMBINANT PROTEINS; TISSUE PLASMINOGEN ACTIVATOR; TRANSCRIPTION, GENETIC; TRANSGENES;

CRICETULUS GRISEUS;

EID: 77952580874     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.03.020     Document Type: Article

References (24)

1. Bode J., Schlake T., Iber M., Schubeler D., Seibler J., Snezhkov E., Nikolaev L. The transgeneticist's toolbox: novel methods for the targeted modification of eukaryotic genomes. Biol. Chem. 2000, 381:801-813.
2. Coroadinha A.S., Schucht R., Gama-Norton L., Wirth D., Hauser H., Carrondo M.J. The use of recombinase mediated cassette exchange in retroviral vector producer cell lines: predictability and efficiency by transgene exchange. J. Biotechnol. 2006, 124:457-468.
3. Fann C.H., Guirgis F., Chen G., Lao M.S., Piret J.M. Limitations to the amplification and stability of human tissue-type plasminogen activator expression by Chinese hamster ovary cells. Biotechnol. Bioeng. 2000, 69:204-212.
4. Gorman C., Bullock C. Site-specific gene targeting for gene expression in eukaryotes. Curr. Opin. Biotechnol. 2000, 11:455-460.
5. Han S.W., Yu W.Y., Li X.Z. Study on plasminogen activators secreted by various cultured cell. Bull. Acad. Military (Med. Sci.) 1987, 11:101-108.
6. Hirata R., Chamberlain J., Dong R., Russell D.W. Targeted transgene insertion into human chromosomes by adeno-associated virus vectors. Nat. Biotechnol. 2002, 20:735-738.
7. Huang Y., Li Y., Wang Y.G., Gu X., Wang Y., Shen B.F. An efficient and targeted gene integration system for high-level antibody expression. J. Immunol. Methods 2007, 322:28-39.
8. Kameyama Y., Kawabe Y., Ito A., Kamihira M. An accumulative site-specific gene integration system using cre recombinase-mediated cassette exchange. Biotechnol. Bioeng. 2010, 105:1106-1114.
9. Kennard M.L., Goosney D.L., Monteith D., Zhang L., Moffat M., Fischer D., Mott J. The generation of stable, high MAb expressing CHO cell lines based on the artificial chromosome expression (ACE) technology. Biotechnol. Bioeng. 2009, 104:540-553.
10. Kim N.S., Kim S.J., Lee G.M. Clonal variability within dihydrofolate reductase-mediated gene amplified Chinese hamster ovary cells: stability in the absence of selective pressure. Biotechnol. Bioeng. 1998, 60:679-688.
11. Kim S.J., Kim N.S., Ryu C.J., Hong H.J., Lee G.M. Characterization of chimeric antibody producing CHO cells in the course of dihydrofolate reductase-mediated gene amplification and their stability in the absence of selective pressure. Biotechnol. Bioeng. 1998, 58:73-84.
12. Kito M., Itami S., Fukano Y., Yamana K., Shibui T. Construction of engineered CHO strains for high-level production of recombinant proteins. Appl. Microbiol. Biotechnol. 2002, 60:442-448.
13. Koduri R.K., Miller J.T., Thammana P. An efficient homologous recombination vector pTV(I) contains a hot spot for increased recombinant protein expression in Chinese hamster ovary cells. Gene 2001, 280:87-95.
14. Koetsier P.A., Schorr J., Doerfler W. A rapid optimized protocol for downward alkaline Southern blotting of DNA. Biotechniques 1993, 15:260-262.
15. Little P. Genetics. small and perfectly formed. Nature 1993, 366:204-205.
16. Lucas B.K., Giere L.M., DeMarco R.A., Shen A., Chisholm V., Crowley C.W. High-level production of recombinant proteins in CHO cells using a dicistronic DHFR intron expression vector. Nucleic Acids Res. 1996, 24:1774-1779.
17. Page M.J., Sydenham M.A. High level expression of the humanized monoclonal antibody Campath-1H in Chinese hamster ovary cells. Biotechnology (NY) 1991, 9:64-68.
18. Reff, M.E., 1998. Impaired dominant selectable marker sequence and intronic inser-tion strategies for enhancement of expression of gene product and expression vector systems comprising same. US Patent No. 5,733,799.
19. Sautter K., Enenkel B. Selection of high-producing CHO cells using NPT selection marker with reduced enzyme activity. Biotechnol. Bioeng. 2005, 89:530-538.
20. Thyagarajan B., Guimaraes M.J., Groth A.C., Calos M.P. Mammalian genomes contain active recombinase recognition sites. Gene 2000, 244:47-54.
21. Weikert S., Papac D., Briggs J., Cowfer D., Tom S., Gawlitzek M., Lofgren J., Mehta S., Chisholm V., Modi N., Eppler S., Carroll K., Chamow S., Peers D., Berman P., Krummen L. Engineering Chinese hamster ovary cells to maximize sialic acid content of recombinant glycoproteins. Nat. Biotechnol. 1999, 17:1116-1121.
22. Wirth D., Gama-Norton L., Riemer P., Sandhu U., Schucht R., Hauser H. Road to precision: recombinase-based targeting technologies for genome engineering. Curr. Opin. Biotechnol. 2007, 18:411-419.
23. Wurm F.M. Production of recombinant protein therapeutics in cultivated mammalian cells. Nat. Biotechnol. 2004, 22:1393-1398.
24. Yoshikawa T., Nakanishi F., Ogura Y., Oi D., Omasa T., Katakura Y., Kishimoto M., Suga K. Amplified gene location in chromosomal DNA affected recombinant protein production and stability of amplified genes. Biotechnol. Prog. 2000, 16:710-715.