Cytogenetic analysis of chimeric antibody-producing CHO cells in the course of dihydrofolate reductase-mediated gene amplification and their stability in the absence of selective pressure

Biotechnology and Bioengineering

Volumn 64, Issue 6, 1999, Pages 741-749

  Kim, Sang Jick ^a   Lee, Gyun Min ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Chromosome; Fluorescence in situ hybridization; Recombinant Chinese hamster ovary (CHO) cells; Stability

Indexed keywords

ANIMAL CELL CULTURE; BIOMARKERS; CHROMOSOMES; CLONING; DNA; ENZYMES; FLUORESCENCE; PRESSURE EFFECTS;

CYTOGENETIC ANALYSIS; FLUORESCENCE IN SITU HYBRIDIZATION (FISH); GENE AMPLIFICATION; REDUCTASE; SOUTHERN BLOT HYBRIDIZATION;

ANTIBODIES;

DIHYDROFOLATE REDUCTASE; METHOTREXATE;

ANIMAL CELL; ANTIBODY PRODUCING CELL; ANTIBODY PRODUCTION; ARTICLE; CELL CLONING; CHO CELL; CHROMOSOME ANALYSIS; GENE AMPLIFICATION; GENE LOCATION; GENETIC STABILITY; IN SITU HYBRIDIZATION; METAPHASE CHROMOSOME; NONHUMAN; SOUTHERN BLOTTING;

ANIMALS; ANTIBODIES; BASE SEQUENCE; BLOTTING, SOUTHERN; CELLS, CULTURED; CHO CELLS; CRICETINAE; DNA, COMPLEMENTARY; GENE AMPLIFICATION; GENETIC MARKERS; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN LIGHT CHAINS; IN SITU HYBRIDIZATION, FLUORESCENCE; METHOTREXATE; RECOMBINANT PROTEINS; SELECTION (GENETICS); TETRAHYDROFOLATE DEHYDROGENASE; TRANSFECTION;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS;

EID: 0344200036     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19990920)64:6<741::AID-BIT14>3.0.CO;2-X     Document Type: Article

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