Use of Flp-mediated cassette exchange in the development of a CHO cell line stably producing erythropoietin

Journal of Microbiology and Biotechnology

Volumn 18, Issue 7, 2008, Pages 1342-1351

  Kim, Min Soo ^a   Lee, Gyun Min ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

EPO producing CHO cell line; Flp mediated cassette exchange; Long term culture; Stable parental clone

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; ERYTHROPOIETIN; RECOMBINASE;

ANIMAL CELL; ARTICLE; BIOTECHNOLOGICAL PRODUCTION; CHO CELL; CONTROLLED STUDY; FLOW CYTOMETRY; FLP MEDIATED CASSETTE EXCHANGE; GENE CASSETTE; GENE EXPRESSION; GENE TARGETING; GENETIC RECOMBINATION; GENOME ANALYSIS; MOLECULAR CLONING; NONHUMAN; PLASMID; PROTEIN SECRETION; TRANSGENE;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; ERYTHROPOIETIN, RECOMBINANT; GENE DOSAGE; GENE EXPRESSION; GENETIC ENGINEERING; INTEGRASES; PLASMIDS; VIRAL PROTEINS;

EID: 50249137032     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: None     Document Type: Article

References (30)

1. Andreas, S., F. Schwenk, B. Kuter-Luks, N. Faust and R. Kuhn. 2002. Enhanced efficiency through nuclear localization signal fusion on phage φC31-integrase: Activity comparison with Cre and FLPe recombinase in mammalian cells. Nucleic Acidv Res. 30: 2299-2306.
2. Barnes, L. M., C. M. Bentley, and A. J. Dickson. 2003. Stability of protein production from recombinant mammalian cells. Biotechnol. Bioeng. 81: 631-639.
3. Belteki G., M. Gertsenstein, D. W. Ow, and A. Nagy. 2003. Site-specific cassette exchange and germline transmission with mouse ES cells expressing φC31 integrase. Nat. Biotechnol. 21: 321-324.
4. Cheng, E. Y., C. Collins, M. Berru, and M. J. Shulman. 2005. A system for precise analysis of transcription-regulating elements of immunoglobulin genes. BMC Biotechnol. 5: 27.
5. Coroadinha, A. S., R. Schucht L. Gama-Norton, D. Wirth, H. Hauser, and M. J. Carrondo. 2006. The use of recombinase mediated cassette exchange in retroviral vector producer cell lines: Predictability and efficiency by transgene exchange. J. Biotechnol. 124: 457-468.
6. Dorer, D. R. and S. Henikoff. 1994. Expansions of transgene repeats cause heterochromatin formation and gene silencing in Drosophila. Cell 77: 993-1002.
7. Emerman, M. and H. M. Temin. 1984. Genes with promoters in retrovirus vectors can be independently suppressed by an epigenetic mechanism. Cell 39: 459-467.
8. Feng, Y. Q., J. Seibler, R. Alami, A. Eisen, K. A. Westerman, P. Leboulch, S. Fiering, and E. E. Bouhassira. 1999. Site-specificchromosomal integration in mammalian cells: Highly efficient CRE recombinase-mediated cassette exchange. J. Mol Biol. 292: 779-785.
9. Freeman, S. M., C. N. Abboud, K. A. Whartenby, C. H. Packman, D. S. Koeplin, F. L. Moolten, and G. N. Abraham. 1993. The 'bystander effect': Tumor regression when a fraction of the tumor mass is genetically modified. Cancer Res. 53: 5274-5283.
10. Fussenegger, M., J. E. Bailey, H. Hauser, and P. P. Mueller. 1999. Genetic optimization of recombinant glycoprotein production by mammalian cells. Trends Biotechnol. 17: 35-42.
11. Garrick, D., S. Fiering, D. I. Martin, and E. Whitelaw. 1998. Repeat-induced gene silencing in mammals. Nat. Genet. 18: 56-59.
12. Hamel, W., L. Magnelli, V. P. Chiarugi, and M. A. Israel, 1996. Herpes simplex virus thymidine kinase/ganciclovir-mediated apoptotic death of bystander cells. Cancer Res. 56: 2697-2702.
13. Hammill, L., J. Welles, and G R. Carson. 2000. The gel microdrop secretion assay: Identification of a low productivity subpopulation arising during the production of human antibody in CHO cells. Cytotechnology 34: 27-37.
14. Jeong, D. W., J. H. Lee, K. H. Kim, and H. J. Lee. 2006. Development of a food-grade integration vector for heterologous gene expression and protein secretion in Lactococcus lactis. J. Microbiol. Biotechnol. 16: 1799-1808.
15. Jun, S. C., M. S. Kim, J. Y. Baik, S. O. Hwang, and G. M. Lee. 2005. Selection strategies for the establishment of recombinant Chinese hamster ovary cell line with dihydrofolate reductase-mediated gene amplification. Appl. Microbiol. Biotechnol. 69: 162-169.
16. Kim, M. S., W. H. Kim, and G M. Lee. 2007. A simple analysis system for the estimation of recombination efficiency using fluorescence-activated cell sorting. J Biolechnol. 127: 373-384.
17. Kim, N. Y., Y. J. Lee, H. J. Kim, J. H. Choi, J. K. Kim, K. H. Chang, J. H. Kim, and H. J. Kim. 2004. Enhancement of erythropoietin production from Chinese hamster ovary (CHO) cells by introduction of the urea cycle enzymes, carbamoyl phosphate synthetase I and ornithine transcarbamylase. J. Microbiol. Biotechnol. 14: 844-851.
18. Kim, S. J., N. S. Kim C. J. Ryu, H. J. Hong, and G. M. Lee. 1998. Characterization of chimeric antibody producing CHO cells in the course of dihydrofolate reductase-mediated gene amplification and their stability in the absence of selective pressure. Biotechnol. Bioeng. 58: 73-84.
19. Kito, M., S. Itami, Y. Fukano, K. Yamana, and T. Shibui. 2002. Construction of engineered CHO strains for high-level production of recombinant proteins. Appl. Microbiol. Biotechnol. 60: 442-448.
20. Koyama. Y, T. Banzai, S. Sonezaki, and K. Kusano. 2006. Stable expression of a heterogeneous gene introduced via gene targeting into the HPRT locus of human fibrosarcoma cells. Biotechnol. Bioeng. 95: 1052-1060.
21. Kwaks, T. H. and A. P. Otte. 2006. Employing epigenetics to augment the expression of therapeutic proteins in mammalian cells. Trends Biotechnol. 24: 137-142.
22. Loyd, M. R., Y. Okamoto, M. S. Randall, and P. A. Ney. 2003. Role of AP1/ NFE2 binding sites in endogenous alpha-globin gene transcription. Blood 102: 4223-4228.
23. Pallavicini, M. G., P. S. DeTeresa, C. Rosette, J. W. Gray, and F. M. Wurm. 1990. Effects of methotrexate on transfected DNA stability in mammalian cells. Mol. Cell. Biol. 10: 401-404.
24. Park, M. 0., K. H. Lim, T. H. Kim, and H. I. Chang. 2007. Characterization of site-specific recombination by the integrase MJ1 from Enterococcal bacteriophage φFC1. J. Microbiol. Biotechnol. 17: 342-347.
25. Pavlovic, G., V. Burrus, B. Gintz, B. Decaris, and G. Guedon. 2004. Evolution of genomic islands by deletion and tandem accretion by site-specific recombination: ICESt1-related elements from Streptococcus thermophilus. Microbiology 150: 759-774.
26. Reddy, S., J. V. DeGregori, H. von Melchner, and H. E. Ruley. 1991. Retrovirus promoter-trap vector to induce lacZ gene fusions in mammalian cells. J. Virol. 65: 1507-1515.
27. Schlake, T. and J. Bode. 1994. Use of mutated FLP recognition target ( FRT) sites for the exchange of expression cassettes at defined chromosomal loci. Biochemistry 33: 12746-12751.
28. Shimizu, T., T. Oishi, A. Omori, A. Sugiura, K. Hirota, H. Aoyama, et al. 2005. Identification of cis-regulatory sequences in the human angiotensinogen gene by transgene coplacement and site-specific recombination. Mol. Cell. Biol. 25: 2938-2945.
29. Wong, E. T., J. L. Kolman, Y. C. Li, L. D. Mesner, W. Hillen, C. Berens, and G. M. Wahl. 2005. Reproducible doxycycline-inducible transgene expression at specific loci generated by Crerecombinase mediated cassette exchange. Nucleic Acids Res. 33: e147.
30. Wurm, F. M. 2004. Production of recombinant protein therapeutics in cultivated mammalian cells. Nat. Biotechnol. 22: 1393-1398.