Toward more efficient protein expression: Keep the message simple

Molecular Biotechnology

Volumn 34, Issue 2, 2006, Pages 151-156

  Kalwy, Stephan ^a   Rance, James ^a   Young, Robert ^a  

^a LONZA AG   (Switzerland)

Author keywords

Chinese hamster ovary; Codon optimization; Glutamine synthetase; mRNA; Transcription; Translation

Indexed keywords

ANTIBODIES; ENZYME KINETICS; GENES; PROTEINS; RNA;

CHINESE HAMSTER OVARY; CODON OPTIMIZATION; GENE OPTIMIZATION; GLUTAMINE SYNTHETASE; PROTEIN PRODUCTION; TRANSCRIPTION;

GENETIC ENGINEERING;

ANTIBODY; GLUTAMATE AMMONIA LIGASE;

ANIMAL CELL; ANTIBODY PRODUCTION; CHO CELL; CONFERENCE PAPER; FEMALE; GENE EXPRESSION; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; HEAVY CHAIN; INTRON; LIGHT CHAIN; NONHUMAN; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; RNA TRANSLATION;

ANIMALS; ANTIBODIES, MONOCLONAL; CHO CELLS; CODON; CRICETINAE; CRICETULUS; GENETIC VECTORS; GLUTAMATE-AMMONIA LIGASE; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN LIGHT CHAINS; INTRONS; MODELS, BIOLOGICAL; PROTEIN BIOSYNTHESIS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; RNA, MESSENGER;

CRICETULUS GRISEUS;

EID: 33845958693     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/MB:34:2:151     Document Type: Conference Paper

References (24)

1. Birch, J. R., Mainwaring, D. O., and Racher, A. J. (2005) Use of the glutamine synthetase (GS) expression system for the rapid development of highly productive mammalian cell processes, in Modern Biopharmaceuticals, (Knäblein, J, ed.), WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim, pp. 809-832.
2. Rotondaro, L., Mele, A., and Rovera, G. (1996) Efficiency of different viral promoters in directing gene expression in mammalian cells: effect of 3′-untranslated sequences. Gene 168, 195-198.
3. Angelichio, M. L., Beck, J. A., Johansen, H., and Ivey-Hoyle, M. (1991) Comparison of several promoters and polyadenylation signals for use in heterologous gene expression in cultured Drosophila cells. Nucleic Acids Res. 19, 5037-5043.
4. Xu, Z. L. Mizuguchi, H., Ishii-Watabe, A., Uchida, E., Mayumi, T., and Hayakawa, T. (2002) Strength evaluation of transcriptional regulatory elements for transgene expression by adenovirus vector. J. Control Release 81, 155-163.
5. Kim, D., Kim, J. D., Baek, K., Yoon, Y., and Yoon, J. (2003) Improved mammalian expression systems by manipulating transcriptional termination regions. Biotechnol. Prog. 19, 1620-1622
6. Benton, T., Chen, T., McEntee, M., et al. (2002) The use of UCOE vectors in combination with a pre-adapted serum free, suspension cell line allows for rapid production of large scale quantities of protein. Cytotechnol. 38, 43-46.
7. Kim, J. -M., Kim, J. -S., Park, D. -H., et al. (2004) Improved recombinant gene expression in CHO cells using matrix attachment regions. J. Biotechnol. 107, 95-105.
8. Girod, P. -A, Zahn-Zabal, M., and Mermod, N. (2005) Use of the chicken lysozyme 5′ matrix attachment region to generate high producer CHO cell lines. Biotechnol. Bioeng. 5, 1-11.
9. Kalwy, S., Rance, J., Norman, A., and Gay, R. (2005) Towards stronger gene expression: a promoter's story. Proc. 19th ESACT Meeting, in press. AU please update ref. 9
10. Smales, C. M., Dinnis, D. M., Stansfield, S. H., et al. (2004) Comparative proteomic analysis of GS-NS0 murine myeloma cell lines with varying recombinant monoclonal antibody production rate. Biotechnol. Bioeng. 88, 474-488.
11. Barnes, L. M., Bentley, C. M., and Dickson, A. J. (2004) Molecular definition of predictive indicators of stable protein expression in recombinant NS0 myeloma cells. Biotechnol. Bioeng. 85, 115-121.
12. Kim, N. S., Kim, S. J., and Lee, G. M. (1998) Clonal variability within dihydrofolate reductase-mediated gene amplified Chinese hamster ovary cells: stability in the absence of selective pressure. Biotechnol. Bioeng. 60, 679-688.
13. Dinnis, D. M. and James, D. C. (2005) Engineering mammalian cell factories for improved recombinant monoclonal antibody production: lessons from nature? Biotechnol. Bioeng. 91, 180-189.
14. Proudfoot, N. J., Furger, A., and Dye, M. J. (2002) Integrating mRNA processing with transcription. Cell 108, 501-512.
15. Haussler, D. (1998) Computational genefinding. Trends Biochem. Sci. Suppl, 12-15.
16. Degroeve, S., De Baets, B., Van de Peer, Y., and Rouzé, P. (2002) Feature subsets selection for splice site prediction. Bioinformatics 18, S75-S83.
17. Lacy-Hulbert, A., Thomas, R., Li, X. P., Lilley, C. E., Coffin, R. S., and Roes, J. (2001) Interruption of coding sequences by heterologous introns can enhance the functional expression of recombinant genes. Gene Ther. 8, 649-653.
18. Graf, F., Deml, L., and Wagner, R. (2004) Codon-optimized genes that enable increased heterologous expression in mammalian cells and elicit efficient immune responses in mice after vaccination of naked DNA. Methods Mol. Med. 94,197-210.
19. Wand, S., Farfan-Arribas, D. J., Shen, S., et al. (2006) Relative contributions of codon usage, promoter efficiency and leader sequence to the antigen expression and immunogenicity of HIV-1 Env DNA vaccine. Vaccine 24, 4531-4540.
20. Scholten, K. B., Kramer, D., Kueter, E. W., et al. (2006) Codon modification of T cell receptors allows enhanced functional expression in transgenic human T cells. Clin. Immunol. 119, 135-145.
21. Whittle, N., Adair, J., Lloyd, C., et al. (1987) Expression in COS cells of a mouse-human chimaeric B72.3 antibody. Protein Eng. 1, 499-505.
22. Bebbington C. R., Renner, G., Thomson, D., King, D., Abrams, D., and Yarranton, G. T. (1992) High-level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable marker. Bio. Technol. 10, 169-175.
23. Schlatter S., Stansfield, S. H, Dinnis, D. M., Racher, A. J., Birch, J. R., and James, D. C. (2005) On the optimal ratio of heavy to light chain genes for efficient recombinant antibody production by CHO cells. Biotechnol. Prog. 21, 122-133.
24. Yoo, E. H., Chintalacharuvu, K. R., Penichet., M. L., and Morrison, S. L. (2002) Myeloma expression systems. J. Immunol. Methods 261, 1-20.