Fast identification of reliable hosts for targeted cell line development from a limited-genome screening using combined φC31 integrase and CRE-Lox technologies

Biotechnology Progress

Volumn 29, Issue 5, 2013, Pages 1307-1315

  Crawford, Yongping ^a   Zhou, Michelle ^a   Hu, Zhilan ^a   Joly, John ^a   Snedecor, Brad ^a   Shen, Amy ^a   Gao, Albert ^b  

^a GENENTECH INC   (United States)

^b Tufts University   (United States)

Author keywords

C31 integrase; Cell line development; CHO cell; CRE; RMCE; Targeted integration

Indexed keywords

CELL LINES; CHO CELL; CRE; INTEGRASE; RMCE;

CELL CULTURE; GENES; INTEGRATION; PRODUCTIVITY; TURNAROUND TIME;

ANTIBODIES;

CRE RECOMBINASE; INTEGRASE;

ANIMAL; BIOTECHNOLOGY; CELL LINE; CHO CELL LINE; COPY NUMBER VARIATION; CRICETULUS; GENE TRANSFER; GENETIC TRANSFECTION; GENETICS; GENOME; GENOMICS; MOLECULAR CLONING; PLASMID; PROCEDURES; TRANSGENE;

ANIMALS; BIOTECHNOLOGY; CELL LINE; CHO CELLS; CLONING, MOLECULAR; CRICETULUS; DNA COPY NUMBER VARIATIONS; GENE TRANSFER TECHNIQUES; GENOME; GENOMICS; INTEGRASES; PLASMIDS; TRANSFECTION; TRANSGENES;

EID: 84886746943     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.1783     Document Type: Article

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