Conformational thermostabilisation of corticotropin releasing factor receptor 1

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Kean, James ^a   Bortolato, Andrea ^a   Hollenstein, Kaspar ^a   Marshall, Fiona H ^a   Jazayeri, Ali ^a  

^a Heptares Therapeutics Ltd   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

(3,6-DIMETHYL-2-(2,4,6-TRIMETHYLPHENOXY)PYRIDIN-4-YL)(1-ETHYLPROPYL)AMINE; AMINOPYRIDINE DERIVATIVE; CORTICOTROPIN RELEASING FACTOR RECEPTOR; CORTICOTROPIN RELEASING FACTOR RECEPTOR 1;

BINDING SITE; CHEMISTRY; GENETICS; HALF LIFE TIME; HEK293 CELL LINE; HUMAN; INVERSE AGONISM; METABOLISM; MOLECULAR DYNAMICS; MUTAGENESIS; PROTEIN STABILITY; PROTEIN TERTIARY STRUCTURE; X RAY CRYSTALLOGRAPHY;

AMINOPYRIDINES; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; DRUG INVERSE AGONISM; HALF-LIFE; HEK293 CELLS; HUMANS; MOLECULAR DYNAMICS SIMULATION; MUTAGENESIS; PROTEIN STABILITY; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, CORTICOTROPIN-RELEASING HORMONE;

EID: 84953923482     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep11954     Document Type: Article

References (50)

1. Langmead, C. J. et al. Identification of novel adenosine A(2A) receptor antagonists by virtual screening. J. Med. Chem. 55, 1904-9 (2012).
2. Kruse, A. C. et al. Muscarinic acetylcholine receptors: novel opportunities for drug development. Nat. Rev. Drug Discov. 13, 549-60 (2014).
3. Tonack, S., Tang, C. & Offermanns, S. Endogenous metabolites as ligands for G protein-coupled receptors modulating risk factors for metabolic and cardiovascular disease. Am. J. Physiol. Heart Circ. Physiol. 304, H501-13 (2013).
4. Hollenstein, K. et al. Insights into the structure of class B GPCRs. Trends Pharmacol. Sci. 35, 12-22 (2014).
5. Parthier, C., Reedtz-Runge, S., Rudolph, R. & Stubbs, M. T. Passing the baton in class B GPCRs: peptide hormone activation via helix induction? Trends Biochem. Sci. 34, 303-10 (2009).
6. Oppermann, M. Chemokine receptor CCR5: insights into structure, function, and regulation. Cell. Signal. 16, 1201-10 (2004).
7. Wettschureck, N. & Offermanns, S. Mammalian G proteins and their cell type specific functions. Physiol. Rev. 85, 1159-204 (2005).
8. Luttrell, L. M. Arrestin pathways as drug targets. Prog. Mol. Biol. Transl. Sci. 118, 469-97 (2013).
9. Sun, Y. et al. Dosage-dependent switch from G protein-coupled to G protein-independent signaling by a GPCR. EMBO J. 26, 53-64 (2007).
10. Fredriksson, R., Lagerström, M. C., Lundin, L.-G. & Schiöth, H. B. The G-protein-coupled receptors in the human genome form five main families. Phylogenetic analysis, paralogon groups, and fingerprints. Mol. Pharmacol. 63, 1256-72 (2003).
11. Doré, A. S. et al. Structure of the adenosine A(2A) receptor in complex with ZM241385 and the xanthines XAC and caffeine. Structure 19, 1283-93 (2011).
12. Katritch, V., Cherezov, V. & Stevens, R. C. Structure-function of the G protein-coupled receptor superfamily. Annu. Rev. Pharmacol. Toxicol. 53, 531-56 (2013).
13. Hollenstein, K. et al. Structure of class B GPCR corticotropin-releasing factor receptor 1. Nature 499, 438-43 (2013).
14. Siu, F. Y. et al. Structure of the human glucagon class B G-protein-coupled receptor. Nature 499, 444-9 (2013).
15. Doré, A. S. et al. Structure of class C GPCR metabotropic glutamate receptor 5 transmembrane domain. Nature 511, 557-62 (2014).
16. Wu, H. et al. Structure of a class C GPCR metabotropic glutamate receptor 1 bound to an allosteric modulator. Science 344, 58-64 (2014).
17. Wang, C. et al. Structure of the human smoothened receptor bound to an antitumour agent. Nature 497, 338-43 (2013).
18. Perrin, M. H. & Vale, W. W. Corticotropin releasing factor receptors and their ligand family. Ann. N. Y. Acad. Sci. 885, 312-28 (1999).
19. Hemley, C. F., McCluskey, A. & Keller, P. A. Corticotropin releasing hormone-a GPCR drug target. Curr. Drug Targets 8, 105-15 (2007).
20. Bale, T. L. & Vale, W. W. CRF and CRF receptors: role in stress responsivity and other behaviors. Annu. Rev. Pharmacol. Toxicol. 44, 525-57 (2004).
21. Naughton, M., Dinan, T. G. & Scott, L. V. Corticotropin-releasing hormone and the hypothalamic-pituitary-adrenal axis in psychiatric disease. Handb. Clin. Neurol. 124, 69-91 (2014).
22. Zhang, G. et al. Pharmacological characterization of a novel nonpeptide antagonist radioligand, (+ /- )-N-[2-methyl-4- methoxyphenyl]-1-(1-(methoxymethyl) propyl)-6-methyl-1H-1,2,3-triazolo[4,5-c]pyridin-4-amine ([3H]SN003) for corticotropin-releasing factor1 receptors. J. Pharmacol. Exp. Ther. 305, 57-69 (2003).
23. Chen, Y. L. et al. 2-aryloxy-4-alkylaminopyridines: discovery of novel corticotropin-releasing factor 1 antagonists. J. Med. Chem. 51, 1385-92 (2008).
24. Couvineau, A. & Laburthe, M. The family B1 GPCR: structural aspects and interaction with accessory proteins. Curr. Drug Targets 13, 103-15 (2012).
25. Pioszak, A. A., Parker, N. R., Suino-Powell, K. & Xu, H. E. Molecular recognition of corticotropin-releasing factor by its G-protein-coupled receptor CRFR1. J. Biol. Chem. 283, 32900-12 (2008).
26. Grace, C. R. R. et al. NMR structure of the first extracellular domain of corticotropin-releasing factor receptor 1 (ECD1-CRF-R1) complexed with a high affinity agonist. J. Biol. Chem. 285, 38580-9 (2010).
27. Kang, H. J., Lee, C. & Drew, D. Breaking the barriers in membrane protein crystallography. Int. J. Biochem. Cell Biol. 45, 636-44 (2013).
28. Ericsson, U. B., Hallberg, B. M. & Detitta, G. T. Thermofluor-based high-throughput stability optimization of proteins for structural studies. 357, 289-298 (2006).
29. Kean, J. et al. Characterization of a CorA Mg2+ transport channel from Methanococcus jannaschii using a Thermofluor-based stability assay. Mol. Membr. Biol. 25, 653-63 (2008).
30. Tate, C. G. A crystal clear solution for determining G-protein-coupled receptor structures. Trends Biochem. Sci. 37, 343-52 (2012).
31. Arachea, B. T. et al. Detergent selection for enhanced extraction of membrane proteins. Protein Expr. Purif. 86, 12-20 (2012).
32. Seddon, A. M., Curnow, P. & Booth, P. J. Membrane proteins, lipids and detergents: not just a soap opera. Biochim. Biophys. Acta 1666, 105-17 (2004).
33. Derewenda, Z. S. The use of recombinant methods and molecular engineering in protein crystallization. Methods 34, 354-63 (2004).
34. Preininger, A. M., Meiler, J. & Hamm, H. E. Conformational flexibility and structural dynamics in GPCR-mediated G protein activation: a perspective. J. Mol. Biol. 425, 2288-98 (2013).
35. Lebon, G., Bennett, K., Jazayeri, A. & Tate, C. G. Thermostabilisation of an agonist-bound conformation of the human adenosine A(2A) receptor. J. Mol. Biol. 409, 298-310 (2011).
36. Robertson, N. et al. The properties of thermostabilised G protein-coupled receptors (StaRs) and their use in drug discovery. Neuropharmacology 60, 36-44 (2011).
37. Miller-Gallacher, J. L. et al. The 2.1 Å resolution structure of cyanopindolol-bound β 1-adrenoceptor identifies an intramembrane Na+ ion that stabilises the ligand-free receptor. PLoS One 9, e92727 (2014).
38. Shibata, Y. et al. Thermostabilization of the neurotensin receptor NTS1. J. Mol. Biol. 390, 262-77 (2009).
39. Söding, J., Biegert, A. & Lupas, A. N. The HHpred interactive server for protein homology detection and structure prediction. Nucleic Acids Res. 33, W244-8 (2005).
40. Krogh, A., Larsson, B., von Heijne, G. & Sonnhammer, E. L. Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J. Mol. Biol. 305, 567-80 (2001).
41. Cormack, B. P., Valdivia, R. H. & Falkow, S. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173, 33-8 (1996).
42. Kawate, T. & Gouaux, E. Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins. Structure 14, 673-81 (2006).
43. Berman, H. M. et al. The Protein Data Bank. Nucleic Acids Res. 28, 235-42 (2000).
44. Martyna, G. J., Tobias, D. J. & Klein, M. L. Constant pressure molecular dynamics algorithms. J. Chem. Phys. 101, 4177 (1994).
45. Dupeux, F., Röwer, M., Seroul, G., Blot, D. & Márquez, J. A. A thermal stability assay can help to estimate the crystallization likelihood of biological samples. Acta Crystallogr. D. Biol. Crystallogr. 67, 915-9 (2011).
46. Grace, C. R. R. et al. Structure of the N-terminal domain of a type B1 G protein-coupled receptor in complex with a peptide ligand. Proc. Natl. Acad. Sci. USA 104, 4858-63 (2007).
47. Grace, C. R. R. et al. NMR structure and peptide hormone binding site of the first extracellular domain of a type B1 G proteincoupled receptor. Proc. Natl. Acad. Sci. USA 101, 12836-41 (2004).
48. Meruelo, A. D., Han, S. K., Kim, S. & Bowie, J. U. Structural differences between thermophilic and mesophilic membrane proteins. Protein Sci. 21, 1746-53 (2012).
49. Sriprapundh, D., Vieille, C. & Zeikus, J. G. Molecular determinants of xylose isomerase thermal stability and activity: analysis of thermozymes by site-directed mutagenesis. Protein Eng. 13, 259-65 (2000).
50. Isberg, V. et al. GPCRDB: an information system for G protein-coupled receptors. Nucleic Acids Res. 42, D422-5 (2014).