Effective Isotope Labeling of Proteins in a Mammalian Expression System

Methods in Enzymology

Volumn 565, Issue , 2015, Pages 289-307

  Sastry, Mallika ^a   Bewley, Carole A ^b   Kwong, Peter D ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^b NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

Author keywords

Adenovirus vector; Glycoproteins; Isotope labeling; Mammalian expression

Indexed keywords

ADENOVIRUS VECTOR; AMINO ACID; CARBON 13; CRE RECOMBINASE; CYTOPLASM PROTEIN; DISULFIDE; GLYCOPROTEIN GP 120; ISOTOPE; MEMBRANE PROTEIN; NITROGEN 15; PROTEIN; VIRUS DNA;

ANIMAL CELL; CELL SELECTION; CHO CELL LINE; CONTROLLED STUDY; DISULFIDE BOND; FEMALE; GENE EXPRESSION SYSTEM; HEK293 CELL LINE; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; ISOTOPE LABELING; LOXP SITE; MAMMAL CELL; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PLAQUE FORMING CELL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN GLYCOSYLATION; PROTEIN PROCESSING; PROTEIN SECRETION; PROTON NUCLEAR MAGNETIC RESONANCE; SHUTTLE VECTOR; TRANSIENT EXPRESSION; TRANSIENT TRANSFECTION; VIRUS; VIRUS GENOME; VIRUS RECOMBINANT; VIRUS REPLICATION; VIRUS STRAIN; ANIMAL; CRICETULUS; GENETICS; MAMMAL; METABOLISM;

ANIMALS; CHO CELLS; CRICETULUS; ISOTOPE LABELING; MAGNETIC RESONANCE SPECTROSCOPY; MAMMALS; PROTEINS;

EID: 84947224325     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/bs.mie.2015.09.021     Document Type: Chapter

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