Expression of human chorionic gonadotropin uniformly labeled with NMR isotopes in Chinese hamster ovary cells: An advance toward rapid determination of glycoprotein structures

Journal of Biomolecular NMR

Volumn 7, Issue 4, 1996, Pages 295-304

  Lustbader, Joyce W ^a   Birken, Steven ^a   Pollak, Susan ^a   Pound, Andrew ^a   Chait, Brian T ^b   Mirza, Urooj A ^b   Ramnarain, Shakuntala ^c   Canfield, Robert E ^a   Brown, Jonathan Miles ^c  

^a Howard Hughes Medical Institute   (United States)

^b ROCKEFELLER UNIVERSITY   (United States)

^c Martek Biosciences Corporation   (United States)

Author keywords

CHO cells; Glycoprotein hormone; hCG; Non radioactive labeling

Indexed keywords

CHORIONIC GONADOTROPIN; CYCLIC AMP; GLYCOPROTEIN; LUTEINIZING HORMONE RECEPTOR; N ACETYLNEURAMINIC ACID; RECOMBINANT PROTEIN; SIALIC ACID DERIVATIVE;

ANIMAL; ARTICLE; BIOSYNTHESIS; CHEMICAL STRUCTURE; CHEMISTRY; CHO CELL; FEMALE; GENETICS; GLYCOSYLATION; HAMSTER; HUMAN; IN VITRO STUDY; METABOLISM; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PREGNANCY; PROTEIN CONFORMATION; RAT;

ANIMALS; CHO CELLS; CHORIONIC GONADOTROPIN; CRICETINAE; CYCLIC AMP; FEMALE; GLYCOPROTEINS; GLYCOSYLATION; HUMANS; MAGNETIC RESONANCE SPECTROSCOPY; MOLECULAR STRUCTURE; N-ACETYLNEURAMINIC ACID; PREGNANCY; PROTEIN CONFORMATION; RATS; RECEPTORS, LH; RECOMBINANT PROTEINS; SIALIC ACIDS;

EID: 0030167995     PISSN: 09252738     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (44)

1. Bellisario, R., Carlsen, R.B. and Bahl, O.P. (1973) J. Biol. Chem., 248, 6796-6809.
2. Birken, S. and Canfield, R.E. (1982a) In Tumor Imaging: The Radioimmunological Detection of Cancer (Eds, Burchiel, S.W. and Rhodes, B.A.), Masson Publishing USA, Inc., New York, NY, pp. 41-52.
3. Birken, S., Canfield, R.E., Agosto, G. and Lewis, J. (1982) Endocrinology, 110, 1555-1563.
4. Birken, S., Gawinowicz-Kolks, M.A., Amr, S., Nisula, B. and Puett, D. (1986) J. Biol. Chem., 261, 10719-10727.
5. Birken, S., Krichevsky, A., O'Connor, J., Lustbader, J. and Canfield, R. (1990) In Glycoprotein Hormones (Eds, Chin, W.W. and Boime, I.), Serono Symposia, Norwell, MA, pp. 45-61.
6. Birken, S., Gawinowicz, M.A., Kardana, A. and Cole, L.A. (1991) Endocrinology, 129, 1551-1558.
7. Birken, S., Chen, Y., Gawinowicz, M.A., Lustbader, J.W., Pollak, S., Agosto, G., Buck, R. and O'Connor, J. (1993) Endocrinology, 133, 1390-1397.
8. Carlsen, R.B., Bahl, O.P. and Swaminathan, N. (1973) J. Biol. Chem., 248, 6810-6827.
9. Chen, H.-C., Shimohigashi, Y., Dufau, M.L. and Catt, K.J. (1982) J. Biol. Chem., 257, 14446-14452.
10. Chowdhury, S. K., Katta, V. and Chait, B.T. (1990) Rapid Commun. Mass Spectrom., 4, 81-87.
11. Daopin, S., Piez, K.A., Ogawa, Y. and Davies, D.R. (1992) Science, 257, 369-373.
12. DeLean, A., Munson, P.J. and Rodbard, D. (1978) Am. J. Physiol., 235, E97-102.
13. Fogh, R.H., Schipper, D., Boelens, B. and Kaptein, R. (1994) J. Biomol. NMR, 4, 123-128.
14. Grzesiek, S. and Bax, A. (1992) J. Magn. Reson., 96, 432-440.
15. Hancock, W.S. (Ed.) (1977) Specifications of Amino Acids, 4th ed., Ajinomoto, Japan, p. 70.
16. Hansen, A.P., Petros, A.M., Mazar, A.P., Pederson, T.M., Rueter, A. and Fesik, S.W. (1992) Biochemistry, 31, 12713-12718.
17. Keene, J.L., Matzuk, M.M., Otani, T., Fauser, B.C.J.M., Galway, A.B., Hseuh, A.J.W. and Boime, I. (1989) J. Biol. Chem., 264, 4769-4775.
18. Keutmann, H.T., Johnson, L. and Ryan, R.J. (1985) Febs Lett., 185, 333-338.
19. Laemmli, U.K. (1970) Nature, 227, 680-685.
20. Lapthorn, A.J., Harris, D.C., Littlejohn, A., Lustbader, J.W., Canfield, R.E., Machin, K.J., Morgan, F.J. and Isaacs, N.W. (1994) Nature, 369, 455-461.
21. Lustbader, J.W., Wu, H., Birken, S., Pollak, S., Gawinowicz Kolks, M.A., Pound, A.M., Austen, D., Hendrickson, W.A. and Canfield, R.E. (1995) Endocrinology, 136, 640-650.
22. Matzuk, M., Keene, J.L. and Boime, I. (1989) J. Biol. Chem., 264, 2409-2414.
23. McDonald, N.Q., Lapatto, R., Murray-Rust, J., Gunning, J., Wlodawer, A., Blundell, T.L. (1991) Nature, 354, 411-414.
24. Mise, T. and Bahl, O.P. (1980) J. Biol. Chem., 255, 8516-8522.
25. Mise, T. and Bahl, O.P. (1981) J. Biol. Chem., 256, 6587-6592.
26. Morgan, F.J., Birken, S. and Canfield, R.E. (1973) Mol. Cell. Biochem., 2, 97-99.
27. Morgan, F.J., Birken, S. and Canfield, R.E. (1975) J. Biol. Chem., 250, 5247-5258.
28. Manjunath, P. and Sairam, M.R. (1983) J. Biol. Chem., 258, 3554-3558.
29. O'Connor, J.F., Birken, S., Lustbader, J.W., Krichevsky, A., Chen, Y. and Canfield, R.E. (1994) Endocr. Rev., 15, 650-683.
30. Oefner, C., D'Arcy, A., Winkler, F.K., Eggimann, B. and Hosang, M. (1992) Embo J., 11, 3921-3926.
31. Osborn, B.L. and Abramson, F.P. (1995) Anal. Biochem., 229, 347-350.
32. Remerowski, M.L., Domke, T., Groenewegen, A., Pepermans, H.A.M., Hilbers, C.W. and Van de Ven, F.J.M. (1994) J. Biomol. NMR, 4, 257-278.
33. Sairam, M.R. and Manjunath, P. (1982) Int. J. Pept. Protein Res., 19, 315-320.
34. Sairam, M.R. and Manjunath, P. (1983) J. Biol. Chem., 258, 445-449.
35. Sairam, M.R., Linggen, J. and Bhargavi, G.N. (1988) Biosci. Rep., 8, 271-278.
36. Sairam, M.R., Linggen, J., Sairam, J. and Bhargavi, G.N. (1990) Biochem. Cell Biol., 68, 889-893.
37. Schlunegger, M.P., Grutter, M.G. (1992) Nature, 358, 430-434.
38. Strickland, T.W. and Puett, D. (1981) Endocrinology, 109, 1933-1942.
39. Weisshaar, G., Hiyama, J. and Renwick, G.C. (1991) Glycobiology, 1, 393-404.
40. Wold, F. (1981) Annu. Rev. Biochem., 50, 783-814.
41. Wold, F. and Moldave, K. (1984) Methods Enzymol., 106, 29-58.
42. Wtay, W., Bolikas, T., Wray, V.P. and Hancock, R. (1981) Anal. Biochem., 118, 197-203.
43. Wu, H., Lustbader, J.W., Liu, Y., Canfield, R.E. and Hendrickson, W.A. (1994) Structure, 2, 545-558.
44. YMC Application No. 10132 Technical Report (1994), YMC Inc., Wilmington, NC.