Generating mammalian stable cell lines by electroporation

Methods in Enzymology

Volumn 529, Issue , 2013, Pages 209-226

  A Longo, Patti ^a   Kavran, Jennifer M ^a   Kim, Min Sung ^a   Leahy, Daniel J ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC G 418; CELL DNA; DIHYDROFOLATE REDUCTASE; INDICATOR; METHOTREXATE; PLASMID DNA;

ANIMAL CELL; ARTICLE; CELL CLONING; CELL DEATH; CELL GROWTH; CELL LINE; CELL SELECTION; CELL SUSPENSION; CELL VOLUME; CENTRIFUGE; ELECTROPORATION; HUMAN; HUMAN CELL; LAMINAR FLOW; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; TRANSFER CELL; TRANSIENT TRANSFECTION;

MAMMALIA;

COLONIES OF CELLS; DILUTE PLASMID DNA; ELECTROPORATION; METHOTREXATE (MTX); PLATING ELECTROPORATED CELLS; PROTOCOL;

ANIMALS; CELL LINE; CHO CELLS; CRICETINAE; CRICETULUS; ELECTROPORATION; GENETIC VECTORS; MAMMALS; TRANSFECTION;

EID: 84883595274     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-418687-3.00017-3     Document Type: Chapter

References (3)

1. G. Urlaub Deletion of the diploid dihydrofolate reductase locus from cultured mammalian cells Cell 33 2 1983 405 412
2. P.J. Southern, and P. Berg Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter Journal of Molecular and Applied Genetics 1 4 1982 327 341
3. M. Wigler Transformation of mammalian cells with an amplifiable dominant-acting gene Proceedings of the National Academy of Sciences of the United States of America 77 6 1980 3567 3570