Transient mammalian cell transfection with polyethylenimine (PEI)

Methods in Enzymology

Volumn 529, Issue , 2013, Pages 227-240

  Longo, Patti A ^a   Kavran, Jennifer M ^a   Kim, Min Sung ^a   Leahy, Daniel J ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL PROTEIN; POLYETHYLENEIMINE;

ADHERENT CELL; ARTICLE; GENETIC TRANSFECTION; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SUSPENSION CELL CULTURE; TRANSIENT EXPRESSION; TRANSIENT TRANSFECTION;

EUKARYOTA; MAMMALIA;

ADHERENT CELLS; HARVEST CELLS; MAMMALIAN CELL TRANSFECTION; POLYETHYLENIMINE (PEI); PROTOCOL; TRANSFECT CELLS; TRANSIENT TRANSFECTIONS;

ANIMALS; CELL CULTURE TECHNIQUES; CELL LINE; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; POLYETHYLENEIMINE; PROTEINS; TRANSFECTION;

EID: 84883580134     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-418687-3.00018-5     Document Type: Chapter

References (2)

1. O. Boussif A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: Polyethylenimine Proceedings of the National Academy of Sciences of the United States of America 92 16 1995 7297 7301
2. N.D. Sonawane, F.C. Szoka Jr., and A.S. Verkman Chloride accumulation and swelling in endosomes enhances DNA transfer by polyamine-DNA polyplexes The Journal of Biological Chemistry 278 45 2003 44826 44831