Identification and characterization of protein glycosylation using specific endo-and exoglycosidases

Methods in Molecular Biology

Volumn 801, Issue , 2012, Pages 189-211

  Magnelli, Paula ^a   Bielik, Alicia ^a   Guthrie, Ellen ^a  

^a NEW ENGLAND BIOLABS   (United States)

Author keywords

Deglycosylation; Glycoprotein; N Glycan; O GlcNAc; O Glycan; O Glycosidase; PNGase F

Indexed keywords

ACETYLGLUCOSAMINIDASE; ALPHA CRYSTALLIN; GLYCOPROTEIN; GLYCOSIDASE; MONOCLONAL ANTIBODY; N ACETYLGLUCOSAMINE; POLYSACCHARIDE; POLYVINYL DERIVATIVE; POLYVINYLIDENE FLUORIDE; RECOMBINANT PROTEIN;

ALPHA CHAIN; ANIMAL; ARTICLE; ARTIFICIAL MEMBRANE; CHEMISTRY; ENZYME SPECIFICITY; GENETICS; GLYCOSYLATION; HELA CELL; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; MOUSE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DENATURATION;

ACETYLGLUCOSAMINE; ACETYLGLUCOSAMINIDASE; ALPHA-CRYSTALLINS; ANIMALS; ANTIBODIES, MONOCLONAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLYCOPROTEIN HORMONES, ALPHA SUBUNIT; GLYCOPROTEINS; GLYCOSIDE HYDROLASES; GLYCOSYLATION; HELA CELLS; HUMANS; MEMBRANES, ARTIFICIAL; MICE; POLYSACCHARIDES; POLYVINYLS; PROTEIN DENATURATION; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

EID: 80555135901     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-352-3_13     Document Type: Article

References (21)

1. Rich JR, Withers SG. Emerging methods for the production of homogeneous human glycoproteins. Nat Chem Biol. 2009; 5(4):206-15.
2. Hossler P, Khattak SF, Li ZJ. Optimal and consistent protein glycosylation in mammalian cell culture. Glycobiology. 2009; 19(9):936-49.
3. Wells L, Vosseller K, Hart GW. Glycosylation of nucleocytoplasmic proteins: signal transduction and O-GlcNAc. Science. 2001, 291(5512):2376-8. (Pubitemid 32231793)
4. Butkinaree C, Park K, Hart GW. O-linked f-N-acetylglucosamine (O-GlcNAc): Extensive crosstalk with phosphorylation to regulate signaling and transcription in response to nutrients and stress. Biochim Biophys Acta. 2010; 1800(2):96-106.
5. Love DC, Hanover JA. The hexosamine signaling pathway: deciphering the "O-GlcNAc code". Sci STKE. 2005; 2005(312): re13.
6. Wang Z, Udeshi ND, Slawson C, Compton PD, Sakabe K, Cheung WD, Shabanowitz J, Hunt DF, Hart GW. Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesis. Sci Signal. 2010, 3(104): ra2.
7. Lazarus BD, Love DC, Hanover JA. O-GlcNAc cycling: implications for neurodegenerative disorders. Int J Biochem Cell Biol. 2009, 41(11):2134-46
8. Bielik AM, Zaia J. Extraction of chondroitin/dermatan sulfate glycosaminoglycans from connective tissue for mass spectrometric analysis. Methods Mol Biol. 2010, 600: 215-25.
9. Azzouz N, Gerold P, Schwarz RT. Metabolic labeling and structural analysis of glycosylphosphatidylinositols from parasitic protozoa. Methods Mol Biol. 2008, 446:183-98.
10. Varki A, Cummings RD, Esko JD, Freeze HH, Stanley P, Bertozzi CR, Hart GW, Etzler ME. (Eds). Essentials of Glycobiology, 2nd ed. 2008. Cold Spring Harbor Laboratory Press. Plainview (NY).
11. Carlsen RB, Bahl OP, Swaminathan N. Human chorionic gonadotropin. Linear amino acid sequence of the beta subunit. J Biol Chem. 1973, 248(19):6810-2.
12. Lectin analysis of proteins blotted onto filters. Freeze HH. Curr Protoc Mol Biol. 2001; Chapter 17:Unit17.7.
13. Thakur D, Rejtar T, Karger BL, Washburn NJ, Bosques CJ, Gunay NS, Shriver Z, Venkataraman G. Profiling the glycoforms of the intact alpha subunit of recombinant human chorionic gonadotropin by high-resolution capillary electrophoresis-mass spectrometry. Anal Chem. 2009, 81(21):8900-7.
14. Comer FI, Vosseller K, Wells L, Accavitti MA, Hart GW. Characterization of a mouse monoclonal antibody specific for O-linked N-acetylglucosamine. Anal Biochem. 2001; 293(2):169-77. (Pubitemid 32547901)
15. Boeggeman E, Ramakrishnan B, Kilgore C, Khidekel N, Hsieh-Wilson LC, Simpson JT, Qasba PK. Direct identification of nonreducing GlcNAc residues on N-glycans of glycoproteins using a novel chemoenzymatic method. Bioconjug Chem. 2007; 18(3):806-14. (Pubitemid 47010801)
16. Roquemore EP, Dell A, Morris HR, Panico M, Reason AJ, Savoy LA, Wistow GJ, Zigler JS Jr, Earles BJ, Hart GW. Vertebrate lens alphacrystallins are modified by O-linked N-acetylglucosamine. Journal of Biological Chem. 1992, 267:555-63.
17. Bielik, A.M. New England Biolabs, Inc., Unpublished Results.
18. Clark PM, Dweck JF, Mason DE, Hart CR, Buck SB, Peters EC, Agnew BJ, Hsieh-Wilson LC. Direct in-gel fluorescence detection and cellular imaging of O-GlcNAc-modified proteins. J Am Chem Soc. 2008; 130(35):11576-7.
19. Kelly WG, Hart GW. Glycosylation of chromosomal proteins: localization of O-linked N-acetylglucosamine in Drosophila chromatin. Cell. 1989; 57(2):243-51. (Pubitemid 19112364)
20. Zachara NE. Detection and analysis of O-linked f-N-acetylglucosamine- modified proteins. Methods Mol Biol. 2009; 464:227-54.
21. Park S, Lee MR, Shin I Carbohydrate microarrays as powerful tools in studies of carbohydrate-mediated biological processes. Chem Commun (Cambridge). 2008;(37):4389-99.