Methods to Construct Recombinant Adenovirus Vectors

Methods in Molecular Biology

Volumn 737, Issue , 2011, Pages 117-138

  Chillon, Miguel ^a   Alemany, Ramon ^b  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^b NONE

Author keywords

Adenovirus construction; Adenovirus genome; Cloning the gene of interest; Homologous recombination

Indexed keywords

RECOMBINANT DNA;

ADENOVIRUS; ARTICLE; CELL STRAIN HEK293; CULTURE TECHNIQUE; ESCHERICHIA COLI; GENE VECTOR; GENETIC TRANSFORMATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; MOLECULAR CLONING; PLASMID; RESTRICTION MAPPING; STANDARD; VIRION; VIROLOGY; YEAST;

ADENOVIRIDAE; CELL CULTURE TECHNIQUES; CLONING, MOLECULAR; DNA, RECOMBINANT; ESCHERICHIA COLI; GENETIC VECTORS; HEK293 CELLS; HUMANS; PLASMIDS; RESTRICTION MAPPING; TRANSFORMATION, GENETIC; VIRION; YEASTS;

EID: 80052329329     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-095-9_5     Document Type: Chapter

References (12)

1. Graham, F. L., and Prevec, L. (1995) Methods for construction of adenovirus vectors Mol. Biotechnol. 3, 207–220.
2. Barratt-Boyes, S. M., Soloff, A. C., Gao, W., Nwanegbo, E., Liu, X., Rajakumar, P. A., et al. (2006) Broad cellular immunity with robust memory responses to simian immunodeficiency virus following serial vaccination with adenovirus 5-and 35-based vectors. J. Gen. Virol. 87, 139–149.
3. Glasgow, J. N., Kremer, E. J., Hemminki, A., Siegal, G. P., Douglas, J. T., and Curiel, D. T. (2004) An adenovirus vector with a chimeric fiber derived from canine adenovirus type 2 displays novel tropism. Virology 324, 103–116.
4. Cascallo, M., Alonso, M. M., Rojas, J. J., Perez-Gimenez, A., Fueyo, J., and Alemany, R. (2007) Systemic toxicity-efficacy profile of ICOVIR-5, a potent and selective oncolytic adenovirus based on the pRB pathway. Mol. Ther. 15, 1607–1615.
5. Alba, R., Hearing, P., Bosch, A., and Chillon, M. (2007) Differential amplification of adenovirus vectors by flanking the packaging signal with attB/attP-PhiC31 sequences: implications for helper-dependent adenovirus production. Virology 367, 51–58.
6. Kremer, E. J., Boutin, S., Chillon, M., and Danos, O. (2000) Canine adenovirus vectors: an alternative for adenovirus-mediated gene transfer. J. Virol. 74, 505–512.
7. Delenda, C., Chillon, M., Douar, A.-M., and Merten, O.-W. (2007) Cells for gene therapy and vector production. Methods in Biotechnology: Animal Cell Biotechnology: Methods and Protocols. Humana Press, (Editor: Ralf Poertner) 24, 23–91.
8. Luo, J., Deng, Z. L., Luo, X., Tang, N., Song, W. X., Chen, J., et al. (2007) A protocol for rapid generation of recombinant adenoviruses using the AdEasy system. Nat. Protoc. 2, 1236–1247.
9. Wu, C., Nerurkar, V. R., Yanagihara, R., and Lu, Y. (2008) Effective modifications for improved homologous recombination and high-efficiency generation of recombinant adenovirus-based vectors. J. Virol. Methods 153, 120–128.
10. Hokanson, C. A., Dora, E., Donahue, B. A., Rivkin, M., Finer, M., and Mendez, M. J. (2003) Hybrid yeast-bacteria cloning system used to capture and modify adenoviral and nonviral genomes. Hum. Gene Ther. 14, 329–339.
11. Burova, E., and Ioffe, E. (2005) Chromatographic purification of recombinant adenoviral and adeno-associated viral vectors: methods and implications. Gene Ther. 12 Suppl 1, S5–S17.
12. Umana, P., Gerdes, C. A., Stone, D., Davis, J. R., Ward, D., Castro, M. G., et al. (2001) Efficient FLPe recombinase enables scalable production of helper-dependent adenoviral vectors with negligible helper-virus contamination. Nat. Biotechnol. 19, 582–585.