메뉴 건너뛰기




Volumn 801, Issue , 2012, Pages 251-268

Optimizing transient recombinant protein expression in mammalian cells

Author keywords

Gateway cloning; Gateway Multisite; HEK293E; Optimizing transient expression; Polyethylenimine

Indexed keywords

POLYETHYLENEIMINE; RECOMBINANT PROTEIN;

EID: 80555135882     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-352-3_16     Document Type: Article
Times cited : (17)

References (11)
  • 1
    • 2342501794 scopus 로고    scopus 로고
    • Transfection of mammalian cells
    • Peel, A., ed. Transfection of mammalian cells, in Methods, vol. 33, No 1, 2004.
    • (2004) Methods , vol.33 , Issue.1
    • Peel, A.1
  • 2
    • 0037082158 scopus 로고    scopus 로고
    • High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells
    • Durocher, Y., Perret, S., and Kamen, A. (2002) High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic Acids Res. 30, 2-9.
    • (2002) Nucleic Acids Res. , vol.30 , pp. 2-9
    • Durocher, Y.1    Perret, S.2    Kamen, A.3
  • 4
    • 0032953248 scopus 로고    scopus 로고
    • Transient gene expression in mammalian cells grown in serum-free suspension culture
    • Schlaeger, E.-J. and Christensen, K. (1999) Transient gene expression in mammalian cells grown in serum-free suspension culture. Cytotechnology 30, 71-83. (Pubitemid 29212107)
    • (1999) Cytotechnology , vol.30 , Issue.1-3 , pp. 71-83
    • Schlaeger, E.-J.1    Christensen, K.2
  • 5
    • 0033676098 scopus 로고    scopus 로고
    • DNA cloning using in vitro site-specific recombination
    • Hartley, J.L., Temple, G.F., and Brasch, M.A. (2000) DNA cloning using in vitro site-specific recombination. Genome Res 10, 1788-95.
    • (2000) Genome Res , vol.10 , pp. 1788-95
    • Hartley, J.L.1    Temple, G.F.2    Brasch, M.A.3
  • 7
    • 7444236455 scopus 로고    scopus 로고
    • Concerted assembly and cloning of multiple DNA segments using in vitro site-specific recombination: Functional analysis of multi-segment expression clones
    • Cheo, D.L., Titus, S.A., Byrd, D.R., Hartley, J.L., Temple G.F., and Brasch, M.A. (2004) Concerted assembly and cloning of multiple DNA segments using in vitro site-specific recombination: functional analysis of multisegment expression clones. Genome Res 14, 2111-20. (Pubitemid 39440193)
    • (2004) Genome Research , vol.14 , Issue.10 B , pp. 2111-2120
    • Cheo, D.L.1    Titus, S.A.2    Byrd, D.R.N.3    Hartley, J.L.4    Temple, G.F.5    Brasch, M.A.6
  • 8
    • 0347753417 scopus 로고    scopus 로고
    • Evidence for high specificity and efficiency of multiple recombination signals in mixed DNA cloning by the Multisite Gateway system
    • DOI 10.1016/j.jbiotec.2003.10.001
    • Sasaki Y, Sone T, Yoshida S, Yahata K, Hotta J, Chesnut JD, Honda T, Imamoto F. (2004) Evidence for high specificity and efficiency of multiple recombination signals in mixed DNA cloning by the Multisite Gateway system. J. Biotechnol. 107, 233-43. (Pubitemid 38091753)
    • (2004) Journal of Biotechnology , vol.107 , Issue.3 , pp. 233-243
    • Sasaki, Y.1    Sone, T.2    Yoshida, S.3    Yahata, K.4    Hotta, J.5    Chesnut, J.D.6    Honda, T.7    Imamoto, F.8
  • 9
    • 49949085914 scopus 로고    scopus 로고
    • Multi-gene gateway clone design for expression of multiple heterologous genes in living cells: Eukaryotic clones containing two and three ORF multi-gene cassettes expressed from a single promoter
    • Sasaki, Y., Sone, T., Yahata K., Kishine H., Hotta, J., Chesnut, J.D., Honda, T., and Imamoto, F. (2008) Multi-gene gateway clone design for expression of multiple heterologous genes in living cells: eukaryotic clones containing two and three ORF multi-gene cassettes expressed from a single promoter. J. Biotechnol. 136, 103-12.
    • (2008) J. Biotechnol. , vol.136 , pp. 103-12
    • Sasaki, Y.1    Sone, T.2    Yahata, K.3    Kishine, H.4    Hotta, J.5    Chesnut, J.D.6    Honda, T.7    Imamoto, F.8
  • 10
    • 0017710978 scopus 로고
    • Characteristics of a human cell line transformed by DNA from human adenovirus type 5
    • Graham, F.L., Smiley, J., Russell, W.C., and Nairn, R. (1977). Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36, 59-74.
    • (1977) J. Gen. Virol. , vol.36 , pp. 59-74
    • Graham, F.L.1    Smiley, J.2    Russell, W.C.3    Nairn, R.4
  • 11
    • 0036618178 scopus 로고    scopus 로고
    • Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells
    • Shaw, G., Morse, S., Ararat, M., and Graham, F.L. (2002) Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells. FASEB J. 16, 869-871.
    • (2002) FASEB J. , vol.16 , pp. 869-871
    • Shaw, G.1    Morse, S.2    Ararat, M.3    Graham, F.L.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.