Mammalian production of an isotopically enriched outer domain of the HIV-1 gp120 glycoprotein for NMR spectroscopy

Journal of Biomolecular NMR

Volumn 50, Issue 3, 2011, Pages 197-207

  Sastry, Mallika ^a   Xu, Ling ^a   Georgiev, Ivelin S ^a   Bewley, Carole A ^b   Nabel, Gary J ^a   Kwong, Peter D ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^b NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

Author keywords

Adenovirus vector; Eukaryotic expression; HIV 1 envelope; Isotope enrichment; N linked glycosylation

Indexed keywords

GLYCOPROTEIN GP 120;

AFFINITY CHROMATOGRAPHY; ARTICLE; CARBON NUCLEAR MAGNETIC RESONANCE; CELL STRAIN HEK293; GLYCOSYLATION; NITROGEN NUCLEAR MAGNETIC RESONANCE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; NUCLEAR OVERHAUSER EFFECT; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTON NUCLEAR MAGNETIC RESONANCE; SIGNAL NOISE RATIO;

ADENOVIRIDAE; CELL LINE, TUMOR; GENETIC VECTORS; HEK293 CELLS; HIV ENVELOPE PROTEIN GP120; HUMANS; MAGNETIC RESONANCE SPECTROSCOPY; SURFACE PLASMON RESONANCE;

ADENOVIRIDAE; BACTERIA (MICROORGANISMS); EUKARYOTA; HUMAN IMMUNODEFICIENCY VIRUS 1; MAMMALIA;

EID: 80051663513     PISSN: 09252738     EISSN: 15735001     Source Type: Journal    
DOI: 10.1007/s10858-011-9506-4     Document Type: Article

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