DULIP: A Dual Luminescence-Based Co-Immunoprecipitation Assay for Interactome Mapping in Mammalian Cells

Journal of Molecular Biology

Volumn 427, Issue 21, 2015, Pages 3375-3388

  Trepte, Philipp ^a   Buntru, Alexander ^a   Klockmeier, Konrad ^a   Willmore, Lindsay ^a   Arumughan, Anup ^a   Secker, Christopher ^a   Zenkner, Martina ^a   Brusendorf, Lydia ^a   Rau, Kirstin ^a   Redel, Alexandra ^a   Wanker, Erich E ^a  

^a MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

Author keywords

detection of low and high affinity interactions; disease mutation detection; luminescence normalization; quantitative interaction score and quantification of interaction strength; systematic protein protein interaction screening

Indexed keywords

FIREFLY LUCIFERASE; MUNC18 PROTEIN; SYNTAXIN 1; PROTEIN BAD; PROTEIN BCL X;

ANALYTIC METHOD; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; DUAL LUMINESCENCE BASED CO IMMUNOPRECIPITATION ASSAY; ENZYME ACTIVITY; HUMAN; HUMAN CELL; MAMMAL CELL; POINT MUTATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PROTEIN INTERACTION; QUALITY CONTROL; QUANTITATIVE ANALYSIS; RELIABILITY; SENSITIVITY AND SPECIFICITY; ANIMAL; CHEMICAL STRUCTURE; GENETICS; HEK293 CELL LINE; IMMUNOPRECIPITATION; LUMINESCENCE; METABOLISM; PROCEDURES;

ANIMALS; BCL-ASSOCIATED DEATH PROTEIN; BCL-X PROTEIN; HEK293 CELLS; HUMANS; IMMUNOPRECIPITATION; LUMINESCENCE; LUMINESCENT MEASUREMENTS; MODELS, MOLECULAR; MUNC18 PROTEINS; POINT MUTATION; PROTEIN INTERACTION MAPPING; SYNTAXIN 1;

EID: 84944256829     PISSN: 00222836     EISSN: 10898638     Source Type: Journal    
DOI: 10.1016/j.jmb.2015.08.003     Document Type: Article

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