High-throughput mapping of a dynamic signaling network in mammalian cells

Science

Volumn 307, Issue 5715, 2005, Pages 1621-1625

  Barrios Rodiles, Miriam ^a   Brown, Kevin R ^b   Ozdamar, Barish ^a,b   Bose, Rohit ^a,b   Liu, Zhong ^a   Donovan, Robert S ^a   Shinjo, Fukiko ^a   Liu, Yongmei ^a   Dembowy, Joanna ^a,b   Taylor, Ian W ^a,b   Luga, Valbona ^a,b   Przulj, Natasa ^c   Robinson, Mark ^c   Suzuki, Harukazu ^d   Hayashizaki, Yoshihide ^d   Jurisica, Igor ^b,c,e   Wrana, Jeffrey L ^a,b  

^a MOUNT SINAI HOSPITAL   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c UNIVERSITY OF TORONTO   (Canada)

^d RIKEN YOKOHAMA INSTITUTE   (Japan)

^e PRINCESS MARGARET HOSPITAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

DISSOLUTION; ENZYMES; GROWTH KINETICS; LUMINESCENCE; POLARIZATION; SIGNALING; THROUGHPUT; TISSUE;

EXTRACELLULAR CUES; MAMMALIAN CELLS; P-21 ACTIVATED KINASE (PAK); SIGNALING NETWORK;

CELLS;

OCCLUDIN; PROTEIN P21; TRANSFORMING GROWTH FACTOR BETA; TRANSFORMING GROWTH FACTOR BETA RECEPTOR; TRANSFORMING GROWTH FACTOR BETA RECEPTOR 1; UNCLASSIFIED DRUG;

PROTEIN;

ARTICLE; CELL COMMUNICATION; CELL POLARITY; CELL TRANSFORMATION; CONTROLLED STUDY; EPITHELIUM CELL; HUMAN; HUMAN CELL; MAMMAL CELL; MESENCHYME CELL; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION; TIGHT JUNCTION;

ACTIVIN RECEPTORS, TYPE I; ANIMALS; CELL LINE; CELL POLARITY; DNA-BINDING PROTEINS; EPITHELIAL CELLS; HUMANS; IMMUNOPRECIPITATION; LUCIFERASES; MEMBRANE PROTEINS; MESODERM; MICE; PHOSPHORYLATION; PROTEIN INTERACTION MAPPING; PROTEIN-SERINE-THREONINE KINASES; RECEPTORS, TRANSFORMING GROWTH FACTOR BETA; RECOMBINANT FUSION PROTEINS; SIGNAL TRANSDUCTION; SMAD2 PROTEIN; SMAD4 PROTEIN; TIGHT JUNCTIONS; TRANS-ACTIVATORS; TRANSFORMING GROWTH FACTOR BETA;

MAMMALIA;

EID: 20144372620     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1105776     Document Type: Article

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26. We thank L. Attisano for critical review and support throughout this project and members of the Attisano and Wrana labs for cDNA reagents, advice, and encouragement throughout this project, especially C. Le Roy, S. Bonni, L. Izzi, and E. Labbé for the Smad subnetwork movie. Work in J.LW.'s lab was supported by funds from Genome Canada, the Natural Sciences and Engineering Research Council (NSERC), the Canadian Institutes of Health Research (CIHR), and the National Cancer Institute of Canada with funds from the Canadian Cancer Society. Work in I.J.'s lab is supported by NIH (P50 CM-62413), NSERC, an International Business Machines (IBM) Shared University Research and IBM Faculty partnership, and from the Institute for Robotics and Intelligent Systems (IRIS). The RIKEN Genomic Sciences Center is supported by the Genome Network Project and the Advanced and Innovational Research Program in Life Science, both from the Ministry of Education, Culture, Sports, Science, and Technology, as well as the Core Research for Evolutional Science and Technology of the Japan Science and Technology Corp. M.B.-R. is a CIHR Postdoctoral Fellow; B.O. and R.B. hold Ph.D. and M.D./Ph.D. CIHR studentships, respectively; K.R.B. is supported by a scholarship from the Institute of Robotics and Intelligent Systems (Precarn Inc.); and N.P. holds an Ontario Graduate Scholarship. J.LW. is an International Scholar of the Howard Hughes Medical Institute and a CIHR Senior Investigator.