Tuning a MAb glycan profile in cell culture: Supplementing N-acetylglucosamine to favour G0 glycans without compromising productivity and cell growth

Journal of Biotechnology

Volumn 214, Issue , 2015, Pages 105-112

  Blondeel, Eric J M ^a   Braasch, Katrin ^b   McGill, Thomas ^a   Chang, David ^c   Engel, Christina ^a   Spearman, Maureen ^b   Butler, Michael ^b   Aucoin, Marc G ^a  

^a UNIVERSITY OF WATERLOO   (Canada)

^b UNIVERSITY OF MANITOBA   (Canada)

^c Chenomx Inc   (Canada)

Author keywords

CHO cells; Glucosamine GlcN; Glycosylation; N acetylglucosamine GlcNAc; Nucleotide sugars; Protein quality

Indexed keywords

ACETYLATION; ANTIBODIES; CELL CULTURE; CELLS; CYTOLOGY; GLUCOSAMINE; GLYCOSYLATION; GROWTH KINETICS; MAMMALS; MONOCLONAL ANTIBODIES; NUCLEOTIDES; POLYSACCHARIDES; PROTEINS; SUPPLY CHAINS;

CHO CELL; GLUCOSAMINE(GLCN); MONOCLONAL ANTIBODIES (MABS); N-ACETYLGLUCOSAMINE; NUCLEOTIDE SUGAR; PROTEIN PRODUCTION; PROTEIN QUALITY; THERAPEUTIC PROTEIN;

CELL GROWTH;

ACETYL COENZYME A; CHIMERIC ANTIBODY; GLUCOSAMINE; GLYCAN; IMMUNOGLOBULIN HEAVY CHAIN; MONOCLONAL ANTIBODY; N ACETYLGLUCOSAMINE; NUCLEOTIDE; SUGAR; CULTURE MEDIUM; GLUCOSE;

ACETYLATION; ANIMAL CELL; ARTICLE; CARBOHYDRATE METABOLISM; CELL GROWTH; CELL VIABILITY; CHO CELL LINE; CONTROLLED STUDY; CULTURE MEDIUM; CULTURE TECHNIQUE; CYTOSOL; FEMALE; GLYCOSYLATION; NONHUMAN; NUCLEOTIDE METABOLISM; PRIORITY JOURNAL; PRODUCTIVITY; STABLE EXPRESSION; ANIMAL; CHEMISTRY; CRICETULUS; HAMSTER; METABOLISM;

ACETYLGLUCOSAMINE; ANIMALS; ANTIBODIES, MONOCLONAL; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA; GLUCOSAMINE; GLUCOSE; GLYCOSYLATION; NUCLEOTIDES;

EID: 84942795126     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2015.09.014     Document Type: Article

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