Metabolic control of recombinant monoclonal antibody N-glycosylation in GS-NS0 cells

Biotechnology and Bioengineering

Volumn 75, Issue 2, 2001, Pages 239-251

  Hills, Anna E ^a   Patel, Ashvin ^b   Boyd, Paul ^a   James, David C ^b  

^a UNIVERSITY OF KENT   (United Kingdom)

^b GLAXOSMITHKLINE   (United Kingdom)

Author keywords

Galactose; Glycosylation; High performance liquid chromatography (HPLC); NS0 murine myeloma cells; Nucleotide sugar

Indexed keywords

CELLS; ENZYMES; METABOLISM; PHARMACOKINETICS; SUGAR (SUCROSE);

METABOLIC CONTROL;

MONOCLONAL ANTIBODIES;

ASPARAGINE; GALACTOSE; GLUCOSAMINE; GLYCAN DERIVATIVE; HEXOSAMINE; IMMUNOGLOBULIN FC FRAGMENT; IMMUNOGLOBULIN G1; MANNOSE; MONOCLONAL ANTIBODY; N ACETYLGLUCOSAMINYLTRANSFERASE; OLIGOSACCHARIDE; RECOMBINANT ANTIBODY;

ARTICLE; CARBOHYDRATE METABOLISM; CELL METABOLISM; CONTROLLED STUDY; GLYCOSYLATION; HUMAN; HUMAN CELL; METABOLIC REGULATION; SIALYLATION; STEREOCHEMISTRY; STRUCTURE ACTIVITY RELATION;

AMIDOHYDROLASES; ANIMALS; ANTHRANILIC ACIDS; ANTIBODIES, MONOCLONAL; CARRIER PROTEINS; CELL ADHESION MOLECULES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CULTURE MEDIA, SERUM-FREE; CYTIDINE MONOPHOSPHATE N-ACETYLNEURAMINIC ACID; GALACTOSE; GLUCOSAMINE; GLYCOSYLATION; HUMANS; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN G; MANNOSE; MEMBRANE PROTEINS; MICE; NUCLEOTIDES; PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL) ASPARAGINE AMIDASE; POLYSACCHARIDES; RECOMBINANT PROTEINS; TUMOR CELLS, CULTURED;

EID: 0035922885     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10022     Document Type: Article

References (49)

1. Functional expression of the murine Golgi CMP-sialic acid transporter in Saccharomyces cerevisiae
2. Nonselective and efficient fluorescent labeling of glycans using 2-aminobenzamide and anthranilic acid
3. Does endogenous glycosylation prevent the use of mouse monoclonal antibodies as cancer therapeutics?
4. The effect of the removal of sialicacid, galactose and total carbohydrate on the functional activity of CAMPATH-1H
5. Intralumenal pool and transport of CMP-N-acetylneuraminic acid, GDP-fucose and UDP-galactose - Study with plasma-membrane-permeabilized mouse thymocytes
6. CMP-N-acetylneuraminic acid: Isolation from and penetration into mouse liver microsome
7. Metabolic shifts do not influence the glycosylation patterns of a recombinant fusion protein expressed in BHK cells
8. Glycobiology - The function of sugar in the IgG molecule
9. Molecular cloning of the hamster CMP-sialic acid transporter
10. One percent of human circulating B-lymphocytes are capable of producing the natural anti-Gal antibody
11. Ammonium ion and glucosamine dependent increases of oligosaccharide complexity in recombinant glycoproteins secreted from cultivated BHK-21 cells
12. Glycosidase activities of the 293 and Ns0 cell lines, and of an antibody-producing hybridoma cell line
13. Intracellular UDP-N-acetylhexosamine pool affects N-glycan complexity: A mechanism of ammonium action on protein glycosylation
14. Improvement of interferon-gamma sialylation in Chinese hamster ovary cell culture by feeding of N-acetylmannosamine
15. A rapid high-resolution high-performance liquid-chromatographic method for separating glycan mixtures and analyzing oligosaccharide profiles
16. Glycosylation of nuclear and cytoplasmic proteins: Purification and characterization of a uridine diphospho-N-acetylglucosamine-polypeptide beta-N-acetylglucos-aminyltransferase
17. Elucidation of the phenotypic change on the surface of Had-1 cell, a mutant-cell line of mouse Fm3a carcinoma cells selected by resistance to Newcastle disease virus infection
18. Crystallographic structure of an intact IgG1 monoclonal antibody
19. O-GlcNAcylation of key nuclear and cytoskeletal proteins - Reciprocity with O-phosphorylation and putative roles in protein multimerization
20. Role of antimetabolites of purine and pyrimidine nucleotide metabolism in tumor cell differentiation
21. Nucleotide sugar transporters: Elucidation of their molecular identity and its implication for future studies
22. Glycosylation and biological activity of CAMPATH-1H expressed in different cell lines and grown under different culture conditions
23. Catabolic control of hybridoma cells by glucose and glutamine limited fed batch cultures
24. Multiple interactions of IgG with its core oligosaccharide can modulate recognition by complement and human Fc-gamma receptor-I and influence the synthesis of its oligosaccharide chains
25. Oligosaccharide protein interactions in IgG can modulate recognition by Fc-γ receptors
26. Fluorophore-labeled carbohydrate analysis of immunoglobulin fusion proteins: Correlation of oligosaccharide content with in vivo clearance profile
27. Pairing of oligosaccharides in the Fc region of immunoglobulin G
28. A systematic approach to the validation of process control parameters for monoclonal antibody production in fed-batch culture of a murine myeloma
29. Mammalian cytidine 5′-monophosphate N-acetylneuraminic acid synthetase: A nuclear protein with evolutionarily conserved structural motifs
30. A high-throughput microscale method to release N-linked oligosaccharides from glycoproteins for matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis
31. A comparative analysis of disease-associated changes: The galactosylation of serum IgG
32. Pyrimidine nucleotide-metabolism in rat hepatocytes - Evidence for compartmentation of nucleotide pools
33. The effect of increasing nucleotide sugar concentrations on the incorporation of sugars into glycoconjugates in rat hepatocytes
34. Species-specific variation in glycosylation of IgG: Evidence for the species-specific sialylation and branch-specific galactosylation and importance for engineering recombinant glycoprotein therapeutics
35. Characterization of a recombinant antibody produced in the course of a high-yield fed-batch process
36. Biochemistry of growth inhibition by ammonium ions in mammalian cells
37. Intracellular ribonucleotide pools as a tool for monitoring the physiological state of in vitro cultivated mammalian cells during production processes
38. Characterization of monoclonal antibody glycosylation: Comparison of expression systems and identification of terminal alpha-linked galactose
39. Differential expression of Gal alpha 1,3Gal epitope in polymeric and monomeric IgM secreted by mouse myeloma cells deficient in alpha 2,6-sialyltransferase
40. Transfer and expression of a murine UDP-Gal-beta-D-Gal-alpha-1,3-galactosyltransferase gene in transfected Chinese hamster ovary cells: Competition reactions between the alpha-1,3-galactosyltransferase and the endogenous alpha-2,3-sialyltransferase
41. Transport of UDP-galactose into the Golgi lumen regulates the biosynthesis of proteoglycans
42. Engineered glycoforms of an antineuroblastoma IgG1 with optimized antibody-dependent cellular cytotoxic activity
43. Incorporation of ammonium into intracellular UDP-activated N-acetylhexosamines and into carbohydrate structures in glycoproteins
44. Engineering Chinese hamster ovary cells to maximize sialic acid content of recombinant glycoproteins
45. Variations in oligosaccharide-protein interactions in immunoglobulin G determine the site-specific glycosylation profiles and modulate the dynamic motion of the Fc oligosaccharides
46. Effect of C2-associated carbohydrate structure on Ig effector function: Studies with chimeric mouse-human IgG1 antibodies in glycosylation mutants of Chinese hamster ovary cells
47. Structural characterization of the N-glycans of a humanized anti-CD8 murine immunoglobulin G
48. Ammonia inhibits neural cell adhesion molecule polysialylation in Chinese hamster ovary and small cell lung cancer cells
49. Role of nucleotide sugar pools in the inhibition of NCAM polysialylation by ammonia