Stable expression of chimeric heavy chain antibodies in CHO cells

Methods in Molecular Biology

Volumn 911, Issue , 2012, Pages 287-303

  Agrawal, Vishal ^a   Slivac, Igor ^a   Perret, Sylvie ^a   Bisson, Louis ^a   St Laurent, Gilles ^a   Murad, Yanal ^a   Zhang, Jianbing ^a,b   Durocher, Yves ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^b UNIVERSITY OF OTTAWA   (Canada)

Author keywords

Antibiotic selection; Antibody production; Cell line development; Fc fusions; PEImax

Indexed keywords

ANTIBIOTIC G 418; BLASTICIDIN S; CHIMERIC ANTIBODY; CHIMERIC HEAVY CHAIN ANTIBODY; HYGROMYCIN B; PHLEOMYCIN; PLASMID VECTOR; POLYETHYLENEIMINE; PUROMYCIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; CHO CELL; CLONING; CONTROLLED STUDY; CULTURE TECHNIQUE; DRUG ACTIVITY; GENETIC TRANSFECTION; HEAVY CHAIN; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SUSPENSION CELL CULTURE; TOXICITY;

EID: 84934435031     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-968-6_18     Document Type: Article

References (14)

1. Zhang J, MacKenzie R, Durocher Y (2009) Production of chimeric heavy-chain antibodies. Methods Mol Biol 525:323-336
2. Hamers-Casterman C et al (1993) Naturally occurring antibodies devoid of light chains. Nature 363:446-448
3. Hmila I et al (2008) VHH, bivalent domains and chimeric heavy chain-only antibodies with high neutralizing efficacy for scorpion toxin AahI'. Mol Immunol 45:3847-3856
4. Bell A et al (2009) Differential tumor-targeting abilities of three single-domain antibody formats. Cancer Lett 289:81-90
5. Durocher Y, Butler M (2009) Expression systems for therapeutic glycoprotein production. Curr Opin Biotechnol 20:700-707
6. Wurm FM (2004) Production of recombinant protein therapeutics in cultivated mammalian cells. Nat Biotechnol 22:1393-1398
7. Urlaub G et al (1983) Deletion of the diploid dihydrofolate reductase locus from cultured mammalian cells. Cell 33:405-412
8. Barnes LM, Bentley CM, Dickson AJ (2003) Stability of protein production from recombi-nant mammalian cells. Biotechnol Bioeng 81: 631-639
9. Pham PL, Kamen A, Durocher Y (2006) Large-scale transfection of mammalian cells for the fast production of recombinant protein. Mol Biotechnol 34:225-237
10. Durocher Y, Perret S, Kamen A (2002) High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic Acids Res 30:E9
11. Loignon M et al (2008) Stable high volumetric production of glycosylated human recombinant IFNalpha2b in HEK293 cells. BMC Biotechnol 8:65
12. Zhang J et al (2009) Transient expression and puri fication of chimeric heavy chain antibodies. Protein Expr Purif 65:77-82
13. Urlaub G, McDowell J, Chasin LA (1985) Use of fluorescence-activated cell sorter to isolate mutant mammalian cells de ficient in an internal protein, dihydrofolate reductase. Somat Cell Mol Genet 11:71-77
14. Allen GC, Spiker S, Thompson WF (2000) Use of matrix attachment regions (MARs) to minimize transgene silencing. Plant Mol Biol 43: 361-376