A novel injectable thermoresponsive and cytocompatible gel of poly(N-isopropylacrylamide) with layered double hydroxides facilitates siRNA delivery into chondrocytes in 3D culture

Acta Biomaterialia

Volumn 23, Issue , 2015, Pages 214-228

  Yang, Hsiao Yin ^a   Van Ee, Renz J ^b   Timmer, Klaas ^b   Craenmehr, Eric G M ^b   Huang, Julie H ^a   Öner, F Cumhur ^a   Dhert, Wouter J A ^a,c   Kragten, Angela H M ^a   Willems, Nicole ^c   Grinwis, Guy C M ^c   Tryfonidou, Marianna A ^c   Papen Botterhuis, Nicole E ^b   Creemers, Laura B ^a  

^a UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

^b TNO   (Netherlands)

^c UTRECHT UNIVERSITY   (Netherlands)

Author keywords

Chondrocytes; Injectable hydrogel; Layered double hydroxides; Poly(N isopropylacrylamide); siRNA delivery

Indexed keywords

ACRYLIC MONOMERS; AMIDES; ENZYME ACTIVITY; GENE EXPRESSION; HISTOLOGY; HYDROGELS; RNA; SCAFFOLDS (BIOLOGY);

CARTILAGINOUS TISSUES; CHONDROCYTES; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; HYBRID HYDROGELS; INJECTABLE HYDROGELS; LAYERED-DOUBLE HYDROXIDES; MG-AL LAYERED DOUBLE HYDROXIDE; POLY(N-ISOPROPYLACRYLAMIDE); SIRNA DELIVERY;

TISSUE;

COLLAGEN TYPE 2; COLLAGENASE 3; DRUG CARRIER; FIBRIN; GLYCERALDEHYDE 3 PHOSPHATE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HYALURONIC ACID; HYDROXIDE; LACTATE DEHYDROGENASE; LAYERED DOUBLE HYDROXIDE; POLY(N ISOPROPYLACRYLAMIDE); SMALL INTERFERING RNA; TYROSINE 3 MONOOXYGENASE; UNCLASSIFIED DRUG; ACRYLIC ACID RESIN; BIOMATERIAL; DELAYED RELEASE FORMULATION; GEL; POLY-N-ISOPROPYLACRYLAMIDE;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; ARTICULAR CARTILAGE; BIOCOMPATIBILITY; CARTILAGE CELL; CARTILAGINOUS TISSUE; CELL DENSITY; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL VIABILITY; CONTROLLED STUDY; DOWN REGULATION; DRUG DELIVERY SYSTEM; ENZYME ACTIVATION; ENZYME ACTIVITY; FLUORESCENCE ACTIVATED CELL SORTING; GEL PERMEATION CHROMATOGRAPHY; GENE EXPRESSION; GENE SILENCING; GENETIC TRANSFECTION; HUMAN; HYDROGEL; MOLECULAR WEIGHT; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; ROOM TEMPERATURE; ADMINISTRATION AND DOSAGE; CELL CULTURE; CHEMISTRY; DELAYED RELEASE FORMULATION; GEL; GENETICS; HEAT; MICROINJECTION; PHYSIOLOGY; PROCEDURES;

ACRYLIC RESINS; BIOCOMPATIBLE MATERIALS; CELLS, CULTURED; CHONDROCYTES; DELAYED-ACTION PREPARATIONS; GELS; HOT TEMPERATURE; HUMANS; HYDROXIDES; MICROINJECTIONS; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 84938211140     PISSN: 17427061     EISSN: 18787568     Source Type: Journal    
DOI: 10.1016/j.actbio.2015.05.018     Document Type: Article

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