Osteogenic differentiation as a result of BMP-2 plasmid DNA based gene therapy in vitro and in vivo

European Cells and Materials

Volumn 21, Issue , 2011, Pages 230-242

  Wegman, F ^a   Bijenhof, A ^a   Schuijff, L ^a   Oner F C ^a   Dhert, W J A ^a,b   Alblas, J ^a  

^a UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

^b UTRECHT UNIVERSITY   (Netherlands)

Author keywords

Bone graft; Bone morphogenetic protein (BMP); Bone regeneration; Hydrogel; Multipotent stromal cells (MSC); Osteogenic differentiation; Transfection

Indexed keywords

ALGINIC ACID; ALKALINE PHOSPHATASE BONE ISOENZYME; BONE MORPHOGENETIC PROTEIN 2; COLLAGEN TYPE 1; GREEN FLUORESCENT PROTEIN; NAKED DNA; OSTEOCALCIN; PLASMID DNA; ALKALINE PHOSPHATASE; DNA; GLUCURONIC ACID; HEXURONIC ACID;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BONE MINERALIZATION; BONE REGENERATION; CELL DIFFERENTIATION; COLLAGEN SYNTHESIS; CONTROLLED STUDY; DRUG DELIVERY SYSTEM; DRUG FORMULATION; ENZYME SYNTHESIS; FEMALE; GENE THERAPY; GENETIC TRANSFECTION; GOAT; HUMAN; HUMAN CELL; HYDROGEL; IN VITRO STUDY; IN VIVO STUDY; MESENCHYMAL STEM CELL TRANSPLANTATION; MOUSE; MULTIPOTENT STEM CELL; NONHUMAN; OSTEOBLAST; PROTEIN SECRETION; ANIMAL; BONE DEVELOPMENT; CYTOLOGY; DRUG EFFECT; ENZYMOLOGY; FLOW CYTOMETRY; GENETICS; IMPLANTATION; MESENCHYMAL STEM CELL; METABOLISM; NUDE MOUSE; PARACRINE SIGNALING; PLASMID; TUMOR CELL LINE;

ALGINATES; ALKALINE PHOSPHATASE; ANIMALS; BONE MORPHOGENETIC PROTEIN 2; CALCIFICATION, PHYSIOLOGIC; CELL DIFFERENTIATION; CELL LINE, TUMOR; DNA; FLOW CYTOMETRY; GENE THERAPY; GLUCURONIC ACID; GOATS; GREEN FLUORESCENT PROTEINS; HEXURONIC ACIDS; HUMANS; MESENCHYMAL STEM CELLS; MICE; MICE, NUDE; OSTEOGENESIS; PARACRINE COMMUNICATION; PLASMIDS; PROSTHESIS IMPLANTATION; TRANSFECTION;

EID: 79960981201     PISSN: 14732262     EISSN: None     Source Type: Journal    
DOI: 10.22203/eCM.v021a18     Document Type: Article

References (38)

1. Arthur A, Zannettino A, Gronthos S (2009) The therapeutic applications of multipotential mesenchymal/ stromal stem cells in skeletal tissue repair. J Cell Physiol 218: 237-245.
2. Audouy SA, de Leij LF, Hoekstra D, Molema G (2002) In vivo characteristics of cationic liposomes as delivery vectors for gene therapy. Pharm Res 19: 1599-1605.
3. Bonadio J, Smiley E, Patil P, Goldstein S (1999) Localized, direct plasmid gene delivery in vivo: Prolonged therapy results in reproducible tissue regeneration. Nat Med 5: 753-759.
4. Chenuet S, Martinet D, Besuchet-Schmutz N, Wicht M, Jaccard N, Bon AC, Derouazi M, Hacker DL, Beckmann JS, Wurm FM (2008) Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection. Biotechnol Bioeng 101: 937-945.
5. Clements BA, Incani V, Kucharski C, Lavasanifar A, Ritchie B, Uludag H (2007) A comparative evaluation of poly-L-lysine-palmitic acid and Lipofectamine 2000 for plasmid delivery to bone marrow stromal cells. Biomaterials 28: 4693-4704.
6. Dass CR (2004) Lipoplex-mediated delivery of nucleic acids: Factors affecting in vivo transfection. J Mol Med 82: 579-591.
7. Duarte AR, Mano JF, Reis RL (2009) Dexamethasoneloaded scaffolds prepared by supercritical assisted phase inversion. Acta Biomater 5: 2054-2062.
8. Gawlitta D, Farrell E, Malda J, Creemers LB, Alblas J, Dhert WJ (2010) Modulating endochondral ossification of multipotent stromal cells for bone regeneration. Tissue Eng Part B Rev 16: 385-395.
9. Gazit D, Turgeman G, Kelley P, Wang E, Jalenak M, Zilberman Y, Moutsatsos I (1999) Engineered pluripotent mesenchymal cells integrate and differentiate in regenerating bone: A novel cell-mediated gene therapy. J Gene Med 1: 121-133.
10. Graham FL, van der Eb AJ (1973) A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52: 456-467.
11. Hengge UR, Dexling B, Mirmohammadsadegh A (2001) Safety and pharmacokinetics of naked plasmid DNA in the skin: Studies on dissemination and ectopic expression. J Invest Dermatol 116: 979-982.
12. Higashi T, Nagamori E, Sone T, Matsunaga S, Fukui K (2004) A novel transfection method for mammalian cells using calcium alginate microbeads. J Biosci Bioeng 97: 191-195.
13. Hosseinkhani H, Hosseinkhani M, Khademhosseini A, Kobayashi H (2007) Bone regeneration through controlled release of bone morphogenetic protein-2 from 3-D tissue engineered nano-scaffold. J Control Release 117: 380-386.
14. Hunt NC, Grover LM (2010) Cell encapsulation using biopolymer gels for regenerative medicine. Biotechnol Lett 32: 733-742.
15. Jordan M, Schallhorn A, Wurm FM (1996) Transfecting mammalian cells: optimization of critical parameters affecting calcium-phosphate precipitate formation. Nucleic Acids Res 24: 596-601.
16. Khan Y, Yaszemski MJ, Mikos AG, Laurencin CT (2008) Tissue engineering of bone: material and matrix considerations. J Bone Joint Surg Am 90 Suppl 1: 36-42.
17. Kofron MD, Laurencin CT (2006) Bone tissue engineering by gene delivery. Adv Drug Deliv Rev 58: 555-576.
18. Kong HJ, Kim ES, Huang YC, Mooney DJ (2008) Design of biodegradable hydrogel for the local and sustained delivery of angiogenic plasmid DNA. Pharm Res 25: 1230-1238.
19. Krebs MD, Salter E, Chen E, Sutter KA, Alsberg E (2010) Calcium phosphate-DNA nanoparticle gene delivery from alginate hydrogels induces in vivo osteogenesis. J Biomed Mater Res A 92: 1131-1138.
20. Lam AP, Dean DA (2010) Progress and prospects: Nuclear import of nonviral vectors. Gene Ther 17: 439-447.
21. Louise C (2006) Nonviral vectors. Methods Mol Biol 333: 201-226.
22. Luo D, Saltzman WM (2000) Synthetic DNA delivery systems. Nat Biotechnol 18: 33-37.
23. Ma B, Zhang S, Jiang H, Zhao B, Lv H (2007) Lipoplex morphologies and their influences on transfection efficiency in gene delivery. J Control Release 123: 184-194.
24. McKay WF, Peckham SM, Badura JM (2007) A comprehensive clinical review of recombinant human bone morphogenetic protein-2 (infuse bone graft). Int Orthop 31: 729-734.
25. Park DJ, Choi JH, Leong KW, Kwon JW, Eun HS (2007) Tissue-engineered bone formation with gene transfer and mesenchymal stem cells in a minimally invasive technique. Laryngoscope 117: 1267-1271.
26. Pelled G, Ben-Arav A, Hock C, Reynolds DG, Yazici C, Zilberman Y, Gazit Z, Awad H, Gazit D, Schwarz EM (2010) Direct gene therapy for bone regeneration: Gene delivery, animal models, and outcome measures. Tissue Eng Part B Rev 16: 13-20.
27. Pietersz GA, Tang CK, Apostolopoulos V (2006) Structure and design of polycationic carriers for gene delivery. Mini Rev Med Chem 6: 1285-1298.
28. Poynton AR, Lane JM (2002) Safety profile for the clinical use of bone morphogenetic proteins in the spine. Spine 27: S40-48.
29. Riley EH, Lane JM, Urist MR, Lyons KM, Lieberman JR (1996) Bone morphogenetic protein-2: Biology and applications. Clin Orthop Relat Res: 39-46.
30. Shields LB, Raque GH, Glassman SD, Campbell M, Vitaz T, Harpring J, Shields CB (2006) Adverse effects associated with high-dose recombinant human bone morphogenetic protein-2 use in anterior cervical spine fusion. Spine 31: 542-547.
31. Shimer AL, Oner FC, Vaccaro AR (2009) Spinal reconstruction and bone morphogenetic proteins: Open questions. Injury 40 Suppl 3: S32-38.
32. Somasundaram C, Tournier I, Feldmann G, Bernuau D (1992) Increased efficiency of gene transfection in primary cultures of adult rat hepatocytes stimulated to proliferate: A comparative study using the lipofection and the calcium phosphate precipitate methods. Cell Biol Int Rep 16: 653-662.
33. Sone T, Nagamori E, Ikeuchi T, Mizukami A, Takakura Y, Kajiyama S, Fukusaki E, Harashima S, Kobayashi A, Fukui K (2002) A novel gene delivery system in plants with calcium alginate micro-beads. J Biosci Bioeng 94: 87-91.
34. Southwood LL, Frisbie DD, Kawcak CE, McIlwraith CW (2004) Delivery of growth factors using gene therapy to enhance bone healing. Vet Surg 33: 565-578.
35. Stevens MM, Marini RP, Schaefer D, Aronson J, Langer R, Shastri VP (2005) In vivo engineering of organs: The bone bioreactor. Proc Natl Acad Sci USA 102: 11450-11455.
36. Stiehler M, Duch M, Mygind T, Li H, Ulrich-Vinther M, Modin C, Baatrup A, Lind M, Pedersen FS, Bunger CE (2006) Optimizing viral and non-viral gene transfer methods for genetic modification of porcine mesenchymal stem cells. Adv Exp Med Biol 585: 31-48.
37. Wittrup A, Sandgren S, Lilja J, Bratt C, Gustavsson N, Morgelin M, Belting M (2007) Identification of proteins released by mammalian cells that mediate DNA internalization through proteoglycan-dependent macropinocytosis. J Biol Chem 282:27897-27904.
38. Wolff JA, Budker V (2005) The mechanism of naked DNA uptake and expression. Adv Genet 54: 3-20.