In vitro analysis of PNIPAAm-PEG, a novel, injectable scaffold for spinal cord repair

Acta Biomaterialia

Volumn 5, Issue 4, 2009, Pages 1046-1055

  Comolli, Noelle ^a,b   Neuhuber, Birgit ^b   Fischer, Itzhak ^b   Lowman, Anthony ^a  

^a Drexel University   (United States)

^b DREXEL UNIVERSITY COLLEGE OF MEDICINE   (United States)

Author keywords

Hydrogel; Injectable; Neurotrophins; Spinal cord injury

Indexed keywords

ACRYLIC MONOMERS; BIOMECHANICS; HYDROGELS; NEURONS; PATIENT REHABILITATION; REPAIR; SCAFFOLDS (BIOLOGY); TISSUE;

COMPRESSIVE MODULI; IN-VITRO ANALYSIS; INJECTABLE SCAFFOLDS; INJECTABLES; MECHANICAL SUPPORT; NEURONAL TISSUES; NEUROTROPHINS; POLY(N-ISOPROPYLACRYLAMIDE); SPINAL CORD INJURY; SPINAL-CORD;

POLYETHYLENE GLYCOLS;

BRAIN DERIVED NEUROTROPHIC FACTOR; MACROGOL; NEUROTROPHIN 3; POLY(N ISOPROPYLACRYLAMIDE); TISSUE SCAFFOLD;

ANIMAL TISSUE; ARTICLE; BIOMECHANICS; CELL ADHESION; CELL SURVIVAL; COMPRESSIVE STRENGTH; CONTROLLED STUDY; EMBRYO; HUMAN; HUMAN CELL; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; SPINAL CORD INJURY; SPINAL CORD SURGERY; STROMA CELL; YOUNG MODULUS;

EID: 64249159628     PISSN: 17427061     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.actbio.2008.10.008     Document Type: Article

References (51)

1. Taylor L., Jones L., Tuszynski M., and Blesch A. Neurotrophin-3 gradients established by lentiviral gene delivery promote short-distance axonal bridging beyond cellular grafts in the injured spinal cord. J Neurosci 26 38 (2006) 9713-9721
2. Piantino J., Burdick J.A., Goldberg D., Langer R., and Benowitz L.I. An injectable, biodegradable hydrogel for trophic factor delivery enhances axonal rewiring and improves performance after spinal cord injury. Exp Neurol 201 2 (2006) 359-367
3. Dhar S., Radulescu D., Gharibjan N., Hayes D.J., and Evans G.R.D. Regulated nerve growth factor delivery on novel polymers by hNGF-EcR-293-TK cells. J Reconstr Microsurg 22 (2006) (Abstract A028)
4. Chen Q., Zhou L., and Shine H.D. Expression of neurotrophin-3 promotes axonal plasticity in the acute but not chronic injured spinal cord. J Neurotrauma 23 8 (2006) 1254-1260
5. Burdick J.A., Ward M., Liang E., Young M.J., and Langer R. Stimulation of neurite outgrowth by neurotrophins delivered from degradable hydrogels. Biomaterials 27 3 (2006) 452-459
6. Mitsui T., Fischer I., Shumsky J.S., and Murray M. Transplants of fibroblasts expressing BDNF and NT-3 promote recovery of bladder and hindlimb function following spinal contusion injury in rats. Exp Neurol 194 2 (2005) 410-431
7. Tuszynski M.H., Grill R., Jones L., Brant A., Blesch A., Low K., et al. NT-3 gene delivery elicits growth of chronically injured corticospinal axons and modestly improves functional deficits after chronic scar resection. Exp Neurol 181 (2003) 47-56
8. Tobias C.A., Shumsky J.S., Shibata M., Tuszynski M.H., Fischer I., Tessler A., et al. Delayed grafting of BDNF and NT-3 producing fibroblasts into the injured spinal cord stimulates sprouting, partially rescues axotomized red nucleus neurons from loss and atrophy, and provides limited regeneration. Exp Neurol 184 1 (2003) 97-113
9. Woerly S. Hydrogels for neural tissue reconstruction and transplantation. Biomaterials 14 14 (1993) 1056-1058
10. Woerly S., Plant G.W., and Harvey A.R. Neural tissue engineering: from polymer to biohybrid organs. Biomaterials 17 3 (1996) 301-310
11. Fischer I., and Seil F.J. Candidate cells for transplantation into the injured CNS. Prog Brain Res (2000) 253-257
12. Hobson M.I., Green C.J., and Terenghi G. VEGF enhances intraneural angiogenesis and improves nerve regeneration after axotomy. J Anat 197 Pt. 4 (2000) 591-605
13. Oudega M., Gautier S.E., Chapon P., Fragoso M., Bates M.L., Parel J.M., et al. Axonal regeneration into Schwann cell grafts within resorbable poly(alpha-hydroxyacid) guidance channels in the adult rat spinal cord. Biomaterials 22 10 (2001) 1125-1136
14. Blesch A., Lu P., and Tuszynski M.H. Neurotrophic factors, gene therapy, and neural stem cells for spinal cord repair. Brain Res Bull 57 6 (2002) 833-838
15. Dalton P.D., Flynn L., and Shoichet M.S. Manufacture of poly(2-hydroxyethyl methacrylate-co-methyl methacrylate) hydrogel tubes for use as nerve guidance channels. Biomaterials 23 18 (2002) 3843-3851
16. Friedman J.A., Windebank A.J., Moore M.J., Spinner R.J., Currier B.L., and Yaszemski M.J. Biodegradable polymer grafts for surgical repair of the injured spinal cord. Neurosurgery 51 3 (2002) 742-751 [discussion 751-742]
17. Geller H.M., and Fawcett J.W. Building a bridge: engineering spinal cord repair. Exp Neurol 174 2 (2002) 125-136
18. Teng Y.D., Lavik E.B., Qu X., Park K.I., Ourednik J., Zurakowski D., et al. Functional recovery following traumatic spinal cord injury mediated by a unique polymer scaffold seeded with neural stem cells. Proc Natl Acad Sci USA 99 5 (2002) 3024-3029
19. Iannotti C., Li H., Yan P., Lu X., Wirthlin L., and Xu X.M. Glial cell line-derived neurotrophic factor-enriched bridgin transplants promote propriospinal axonal regeneration and enhance myelination after spinal cord injury. Exp Neurol 183 (2003) 379-393
20. Schmidt C., and Leach J.B. Neural tissue engineering: strategies for repair and regeneration. Ann Rev Biomed Eng 5 (2003) 293-347
21. Lepore A.C., Bakshi A., Swanger S.A., Rao M.S., and Fischer I. Neural precursor cells can be delivered into the injured cervical spinal cord by intrathecal injection at the lumbar cord. Brain Res. 1045 1-2 (2005) 206-216
22. Neuhuber B., Timothy Himes B., Shumsky J.S., Gallo G., and Fischer I. Axon growth and recovery of function supported by human bone marrow stromal cells in the injured spinal cord exhibit donor variations. Brain Res 1035 1 (2005) 73-85
23. Zhang N., Yan H., and Wen X. Tissue-engineering approaches for axonal guidance. Brain Res Rev 49 1 (2005) 48-64
24. Klapka N., and Muller H.W. Collagen matrix in spinal cord injury. Neurotrauma 23 (2006) 422-435
25. Samadikuchaksaraei A. An overview of tissue engineering approaches for management of spinal cord injuries. J Neuroeng Rehabil 4 15 (2007)
26. Heller J., and Himmelstein K.J. Poly(ortho ester) biodegradable polymer systems. Methods Enzymol 112 (1985) 422-436
27. Shive M.S., and Anderson J.M. Biodegradation and biocompatibility of PLA and PLGA microspheres. Adv Drug Deliv Rev 28 1 (1997) 5-24
28. Hoffman A.S. Hydrogels for biomedical applications. Ann NY Acad Sci 944 (2001) 62-73
29. Lavik E., Teng Y.D., Snyder E., and Langer R. Seeding neural stem cells on scaffolds of PGA PLA, and their copolymers. Methods Mol Biol 198 (2002) 89-97
30. Langer R., and Tirrell D.A. Designing materials for biology and medicine. Nature 428 6982 (2004) 487-492
31. Novikova L., Mosahebi A., Wiberg M., Terenghi G., Kellerth J.O., and Novikova L.N. Alginate hydrogel and matrigel as potential cell carriers for neurotransplantation. J Biomed Mater Res 77 (2006) 242-252
32. Bakshi A., Fisher O., Dagci T., Himes B.T., Fischer I., and Lowman A. Mechanically engineered hydrogel scaffolds for axonal growth and angiogenesis after transplantation in spinal cord injury. J Neurosurg 1 3 (2004) 322-329
33. Sung H.J., Johnson C., and Galis Z. The effect of scaffold degradation rate on three-dimensional cell growth and angiogenesis. Biomaterials 25 26 (2004) 5735-5742
34. Yoshioka H., Mori Y., Tsukikawa S., and Kubota S. Thermoreversible gelation on cooling and on heating of an aqueous gelatin-poly(N-isopropylacrylamide) conjugate. Polym Adv Technol 9 (1998) 155-158
35. Huang X., and Lowe T.L. Biodegradable thermoresponsive hydrogels for aqueous encapsulation and controlled release of hydrophilic model drugs. Biomacromolecules 6 (2005) 2131-2139
36. Lin H., and Cheng Y. In-situ thermoreversible gelation of block and star copolymers of poly(ethylene glycol) and poly(N-isopropylacrylamide) of varying architectures. Macromolecules 34 (2001) 3710-3715
37. Choa J.H., Kimb S., Parkc K.D., Jungc M.C., Yangd W.I., Hane S.W., et al. Chondrogenic differentiation of human mesenchymal stem cells using a thermosensitive poly(N-isopropylacrylamide) and water-soluble chitosan copolymer. Biomaterials 25 (2004) 5743-5751
38. Hoffman A.S. Applications of thermally reversible polymers and hydrogels in therapeutics and diagnostics. J Control Release 6 (1987) 297-305
39. Ozawa H., Matsumoto T., Ohashi T., Sato M., and Kokubun S. Comparison of spinal cord gray matter and white matter softness: measurement by pipette aspiration method. J Neurosurg 95 2 Suppl. (2001) 221-224
40. Ozawa H., Matsumoto T., Ohashi T., Sato M., and Kokubun S. Mechanical properties and function of the spinal pia mater. J Neurosurg 1 1 (2004) 122-127
41. Liu Y., Himes B.T., Solowska J., Moul J., Chow S.Y., Park K.I., et al. Intraspinal delivery of neurotrophin-3 using neural stem cells genetically modified by recombinant retrovirus. Exp Neurol 158 1 (1999) 9-26
42. Liu Y., Kim D., Himes B.T., Chow S.Y., Schallert T., Murray M., et al. Transplants of fibroblasts genetically modified to express BDNF promote regeneration of adult rat rubrospinal axons and recovery of forelimb function. J Neurosci 19 11 (1999) 4370-4387
43. Lombello C.B., Malmonge S.M., and Wada M.L. Morphology of fibroblastic cells cultured on poly(HEMA-co-AA) substrates. Cytobios 101 397 (2000) 115-122
44. Murray M., Kim D., Liu Y., Tobias C., Tessler A., and Fischer I. Transplantation of genetically modified cells contributes to repair and recovery from spinal injury. Brain Res 40 1-3 (2002) 292-300
45. Wu S., Suzuki Y., Kitada M., Kitaura M., Kataoka K., Takahashi J., et al. Migration, integration, and differentiation of hippocampus-derived neurosphere cells after transplantation into injured rat spinal cord. Neuroscience Lett. 312 3 (2001) 173-176
46. KS Lee.J., Shichinohe H., Ikeda J., Seki T., Hida K., et al. Migration and differentiation of nuclear fluorescence-labeled bone marrow stromal cells after transplantation into cerebral infarct and spinal cord injury in mice. Neuropathology 23 (2003) 169-180
47. Wu S., Suzuki Y., Ejiri Y., Noda T., Bai H., Kitada M., et al. Bone marrow stromal cells enhance differentiation of coculture neurosphere cells and promote regeneration of injured spinal cord. J Neurosci Res 72 (2003) 343-351
48. Bryant S. Crosslinking density influences chondrocyte metabolism in dynamically loaded photocrosslinked poly(ethylene glycol) hydrogels. Ann Biomed Eng 32 3 (2003) 407-417
49. Vernengo A.J., Fussell G., Smith N.G., and Lowman A.M. Evaluation of novel injectable hydrogels for nucleus pulposus replacement. J Biomed Mater Res 84b (2008) 64-69
50. Spiller K.L., Laurencin S.J., Charlton D., Maher S.A., and owman A.M. Superporous hydrogels for cartilage repair: evaluation of the morphological and mechanical properties. Acta Biomater 4 1 (2008) 17-25
51. Shibayama M., Himes B.T., Murray M., and Tessler A. Neurotrophin-3 prevents death of axotomized Clarke's nucleus neurons in adult rat. J Comp Neurol 390 1 (1997) 102-111