Cell type and transfection reagent-dependent effects on viability, cell content, cell cycle and inflammation of RNAi in human primary mesenchymal cells

European Journal of Pharmaceutical Sciences

Volumn 53, Issue 1, 2014, Pages 35-44

  Yang, Hsiao Yin ^a   Vonk, Lucienne A ^a   Licht, Ruud ^a   Van Boxtel, Antonetta M G ^a   Bekkers, Joris E J ^a   Kragten, Angela H M ^a   Hein, San ^b   Varghese, Oommen P ^c   Howard, Kenneth A ^b   Cumhur Oner F ^a   Dhert, Wouter J A ^a,d   Creemers, Laura B ^a  

^a UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

^b AARHUS UNIVERSITY   (Denmark)

^c UPPSALA UNIVERSITY   (Sweden)

^d UTRECHT UNIVERSITY   (Netherlands)

Author keywords

Chondrocytes; MSCs; Non specific effects; Nucleus pulposus; siRNA; Transfection

Indexed keywords

CHITOSAN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HYALURONIC ACID; LIPOFECTAMINE; POLYETHYLENEIMINE; POLYSACCHARIDE; REAGENT; SMALL INTERFERING RNA;

ARTICLE; CARTILAGE CELL; CELL CYCLE; CELL CYCLE PROGRESSION; CELL PROLIFERATION; CELL TYPE; CELL VIABILITY; COMPARATIVE STUDY; CONTROLLED STUDY; CYTOTOXICITY; DNA CONTENT; ENZYME ACTIVITY; GENE EXPRESSION; GENE SILENCING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; INFLAMMATION; MESENCHYMAL STEM CELL; MESENCHYME CELL; NUCLEUS PULPOSUS; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RNA INTERFERENCE; UPREGULATION;

CHONDROCYTES; MSCS; NON-SPECIFIC EFFECTS; NUCLEUS PULPOSUS; SIRNA; TRANSFECTION;

AGGRECANS; CARTILAGE, ARTICULAR; CELL CYCLE; CELL SURVIVAL; CELLS, CULTURED; CHITOSAN; CHONDROCYTES; COLLAGEN TYPE I; COLLAGEN TYPE II; CORE BINDING FACTOR ALPHA 1 SUBUNIT; CYCLIN-DEPENDENT KINASE INHIBITOR P21; CYCLOOXYGENASE 2; GENE EXPRESSION; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (PHOSPHORYLATING); HUMANS; HYALURONIC ACID; INFLAMMATION; INTERVERTEBRAL DISC; KNEE JOINT; LIPIDS; LUMBAR VERTEBRAE; MESENCHYMAL STROMAL CELLS; OSTEOPONTIN; POLYETHYLENEIMINE; PROLIFERATING CELL NUCLEAR ANTIGEN; RNA INTERFERENCE; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 84891696095     PISSN: 09280987     EISSN: 18790720     Source Type: Journal    
DOI: 10.1016/j.ejps.2013.12.006     Document Type: Article

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